中国比较医学杂志2017，Vol.27Issue(8)：40-42,3.DOI:10.3969.j.issn.1671-7856.2017.08.008
PKH26和Hoechst 33258联合示踪Uncv无毛小鼠iRhom2及其突变体蛋白
PKH26 combined with Hoechst 33258 to trace the iRhom2 gene and its mutant proteins of Uncv mice in Vero cells
摘要
Abstract
Objective To determine the localization of iRhom2 and its mutant proteins of Uncv mice in Vero cells by PKH26 combined with Hoechst 33258 staining.Methods The cell membrane was stained with PKH26, and the nuclei were stained with Hoechst 33258 dye, and observed by laser scanning confocal microscopy.Results It was found that wild iRhom2 was distributed in the cytoplasm, and its iRhom2mut was present both in cytoplasm and cell nuclei.Conclusions The results of our study suggest that a deletion in N-terminal of iRhom2 affects its subcellular localization.关键词
PKH26/Hoechst33258/iRhom2/定位Key words
PKH26/Hoechst 33258/iRhom2/localization/Vero cells/Subcelluoar localization/Uncv mice分类
医药卫生引用本文复制引用
游颖,陈丙波,曾林..PKH26和Hoechst 33258联合示踪Uncv无毛小鼠iRhom2及其突变体蛋白[J].中国比较医学杂志,2017,27(8):40-42,3.基金项目
国家自然科学基金重点项目(31030058) （31030058）
国家科技支撑计划(2011BA115B03). （2011BA115B03）
