中国比较医学杂志2017，Vol.27Issue(8)：43-49,7.DOI:10.3969.j.issn.1671-7856.2017.08.009
微卫星DNA分析国内24个近交系小鼠遗传状况
Genetic quality analysis of 24 domestic inbred mouse strains by microsatellite DNA
摘要
Abstract
Objective To analyze the genetic quality of 24 domestic inbred strains mice using microsatellite loci panel.Methods Previously selected 30 microsatellite loci of mouse with high polymorphism and more allele numbers were used to synthesize corresponding fluorescently-labeled primers.Then the genomic DNA samples of each mouse were amplified by PCR and the products were analyzed by STR scanning to genotype the inbred strains of mice.Results Out of the 24 inbred strains, 15 inbred strains showed the same genotype within one strain at 30 loci.Among different strains, microsatellite loci indicated polymorphism which could be used to distinguish different strains.However, the rest 9 strains demonstrated polymorphism within strains.Conclusions Our stuoly provides a useful microsatellite panel to detect genetic quality of inbred mice and distinguish different strains with the optimized microsatellite loci.关键词
近交系小鼠/遗传质量分析/微卫星DNAKey words
Inbred strains/Mice/Genetic quality/Microsatellite DNA分类
医药卫生引用本文复制引用
李银银,陈振文,杜小燕,吴绍亮,王洪,萧晓琴,张双悦,郭萌,李长龙,吕建祎,刘欣..微卫星DNA分析国内24个近交系小鼠遗传状况[J].中国比较医学杂志,2017,27(8):43-49,7.基金项目
国家科技支撑计划(2015BAI07B01) （2015BAI07B01）
国家自然科学基金(31372272,31572341). （31372272,31572341）
