中国比较医学杂志2017，Vol.27Issue(8)：60-65,6.DOI:10.3969.j.issn.1671-7856.2017.08.012
肝癌人源肿瘤异种移植模型构建及初步应用
Establishment and application of a patient derived xenograft mouse model of liver cancer
摘要
Abstract
Objective To establish a patient-derived xenografts (PDX) mouse model of liver cancer (LC) and to explore its role in precision medicine.Methods PDX model was established by subcutaneous implantation of tumor tissues in NCG mice.The morphological structure of tumor tissue was exaimed using HE staining.Fifteen BALB/c nude mice were subcutaneously inoculated with tumor cell suspension from the PDX models.The xenograft mice were randomly divided into 5-fluorouracil (5-FU) group, sorafenib group and negative control group.The tumor volume and body weight of the tumor-bearing mice were measured regularly, the tumor inhibition rate was calculated and the curative effect was evaluated.Results The success rate was 33.3% (6/18) in the establishment of liver cancer PDX mouse model, and the model well retained the characteristics of the primary tumor.In one case of PDX mouse model, the tumor inhibition rates of 5-FU and sorafenib group were 63.7% and 29.6%, with a statistically significant differece between them (P< 0.05), and there was no significant difference between the sorafenib group and negative control group, consistent with clinical observation.Conclusions The PDX mouse model of liver cancer can maintain the histological structure of primary tumor, and can be applied to precision medicine for patients with liver cancer.关键词
人源性肿瘤异种移植模型/肝癌/精准医疗Key words
Patient derived xenograft/PDX/Mouse model/Liver cancer/Precision medicine分类
医药卫生引用本文复制引用
黄昊,高苒,李宝亮,杨星九,李梦媛,朱瑞敏,胡娟娟,韦荣飞,李克娟,蒋力..肝癌人源肿瘤异种移植模型构建及初步应用[J].中国比较医学杂志,2017,27(8):60-65,6.基金项目
中国医学科学院医学与健康科技创新工程经费资助(2016-I2M-3-019) （2016-I2M-3-019）
中央级公益性科研院所基本科研业务费(2016ZX310032) （2016ZX310032）
协和青年科研基金资助(3332016078). （3332016078）
