浙江医学2017，Vol.39Issue(13)：1064-1067,1071,5.DOI:10.12056/j.issn.1006-2785.2017.39.13.2017-394
葛瑞林对慢性心力衰竭大鼠心肌组织及血液中TRX和ASK1表达的影响
Effect of Ghrelin on expression of TRX and ASK1 in myocardium and blood of chronic heart failure rats
摘要
Abstract
Objective To investigate the effect of Ghrelin on the expression of thioredoxin (TRX) and apoptotic signal kinase 1 (ASK1) in myocardium and blood of chronic heart failure (CHF) rats.Methods CHF was induced by ligating the left anterior descending coronary artery in 60 male SD rats,then the CHF rats were randomly divided into two groups with 30 animals in each.CHF rats in study group were treated with intravenous injection of Ghrelin;those in control group were treated with intravenous injection of normal saline.The myocardium morphology and ultrastructure were observed by light and electron microscopy on d2,d20 and d45 after CHF induced;left ventricular function was evaluated by polygraphy;expression of TRX and ASK1 proteins in myocardial tissue were detected by immunohistochemistry;the expression of TRX and ASK1 mRNA was detected by RT-PCR.Results The myocardial injury of rats on d2,d20 and d45 after modeling in control group was more severe than that in study group.Electron microscopy also showed that the myocardial damage on d45 in control group was more severe than that in study group.(P＜0.05);the left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) in study group were higher than those in the control group (P＜0.05).The immunohistochemical ASK1 positive area and the expression ASK1 mRNA in myocardia tissue on d45 in the study group were lower than those in the control group,wile the immunohistochemical TRX positve area and the expression of TRX mRNA in myocardial tissue were significantly higher than those in the control group (P＜0.05).Conclusion Ghrelin can downregulate ASK1 expression and upregulate TRX expression in CHF myocardium,and decrease the content of ASK1 and increase the content of TRX in the blood,so as to improve the biological function of CHF.关键词
心力衰竭/心肌/病理学/心肌细胞/细胞凋亡Key words
Heart failure/Myocardium/Pathology/Cardiac muscle cells/Apoptosis引用本文复制引用
徐建博,张茹冰,陈颖,陈哲,刘隽东,陈振栋..葛瑞林对慢性心力衰竭大鼠心肌组织及血液中TRX和ASK1表达的影响[J].浙江医学,2017,39(13):1064-1067,1071,5.基金项目
黑龙江省教育厅基础研究类(自然类)面上项目(JMSUJCM2016-048) （自然类）
