浙江医学2017，Vol.39Issue(13)：1072-1076,1096,后插2,7.DOI:10.12056/j.issn.1006-2785.2017.39.13.2017-404
紫铆因通过自噬途径促进骨肉瘤细胞凋亡及机制研究
Butein inducesapoptosis of human osteosarcoma cells through autophagy pathway
摘要
Abstract
Objective To investigate the effect of butein on apoptosis and autophagy of osteosarcoma MG63 cells and its mechanism.Methods Human osteosarcoma MG63 cells were treated with butein at different concentrations for various times.Cell viability was measured by CCK-8 assay,the expression of apoptosis-related proteins Bax,BcI-2,Cleaved caspase3 and autophagy-related proteins p62,LC3 and Beclin-1 were detected by Western blot,the apoptosis of MG63 cells waere detected by TUNEL staining,the production of autophagic bubbles was observed by transmission electron microscopy.The osteosarcoma MG63 cells were pretreated with autophagy agonists and autophagy inhibitors before butein was added for treatment,the above exprements were repeated to explore the role of autophagy in anti-osteosarcoma of butein.Results After treatment with butein,the viability of MG63 cells were decreased significantly.Western blot results showed that the expression of Bax,Cleaved caspase3,Beclin-1 and LC3 Ⅱ were increased,while the expression of Bcl-2 and p62 were decreased.The results of TUNEL staining showed that there was significant apoptosis in osteosarcoma cells and many autophagic bubbles were observed with transmission electron microscopy in the butein treatment group.Pretreatment of osteosarcoma cells with autophagy inhibitor,the effect of butein on the apoptosis of osteosarcoma was reversed,while pretreatment of osteosarcoma cells with autophagy agonists,the effect of butein on the apoptosis of osteosarcoma was further enhanced.Conclusion Butein can inhibit the viability and induce apoptosis of osteosarcoma MG63 cells,which is associated with the activation of autophagy.关键词
紫铆因/骨肉瘤/凋亡/自噬Key words
Butein/Osteosarcoma/Autophagy/Apoptosis引用本文复制引用
蔡宁宇,潘天龙,李斌,吴登颖,潘骏..紫铆因通过自噬途径促进骨肉瘤细胞凋亡及机制研究[J].浙江医学,2017,39(13):1072-1076,1096,后插2,7.基金项目
温州市科技计划项目(Y20150066) （Y20150066）
