浙江医学2017，Vol.39Issue(14)：1151-1154,4.DOI:10.12056/j.issn.1006-2785.2017.39.14.2017-802
青蒿琥酯诱导胃癌BGC-823细胞死亡的实验研究
Effects of artesunate on transplanted human gastric cancer BGC-823 in nude mice
摘要
Abstract
Objective To investigate the effect of artesunate on transplanted human gastric cancer in nude mice.Methods Human gastric cancer BGC-823 cells were inoculated in nude mice.The tumor-bearing nude mice were treated with artesunate at a dose of 60,120,or 240 mg/ (kg·d),i.p.for 15 d.The changes of body weights and tumor size were observed.The expression of VEGF and Calpain2 mRNA and proteins were detected by RT-PCR,immunohistochemical and Western Blot,respectively.Results Artesunate suppressed BGC-823 tumor growth in nude mice in a dose-dependent manner.The expression of VEGF mRNA and protein were decreased and the expression of Calpain2 mRNA and protein were increased in cancer cells of artesunate-treated nude mice,compared to control group (P ＜0.05).Conclusion Artesunate induces a dose-dependent suppression of BGC-823 tumor growth in nude mice,which might be related to the down-regulation of VEGF and the up-regulation of Calpain2.关键词
青蒿琥酯/胃癌/裸鼠模型Key words
Artesunate/Stomach neoplasms/Nude mice model引用本文复制引用
王维明,周香,郑志强..青蒿琥酯诱导胃癌BGC-823细胞死亡的实验研究[J].浙江医学,2017,39(14):1151-1154,4.基金项目
温州市科技计划项目(Y20140105、Y20140111) （Y20140105、Y20140111）
