浙江医学2017，Vol.39Issue(14)：1155-1157,1174,4.DOI:10.12056/j.issn.1006-2785.2017.39.14.2016-2035
槐耳清膏联合顺铂改变胃癌SGC7901细胞周期分布的实验研究
Combined effects of fungi Huaier extracts and cis-platin on cell cycle of human gastric cancer cell line SGC7901
摘要
Abstract
Objective To investigate the combined effects of fungi Trametes robiniophila murr (Huaier) extracts and cis-platin (DDP) on cell cycle of gastric cancer cell line SGC7901in vitro and the underline mechanism.Methods Human gastric cancer SGC7901 cells were treated with Huaier and/or DDP in vitro.The cell cycle was examined by flow cytometry;and the expression of cell cycle-related proteins was detected by Western blot.Results The ratio of cells in G2/M phase of SGC7901 cells treated with Huaier were increased in a dose-dependent manner compared with control group;and the ratio of cells in S phase in DDP-treated groups were increased also in a dose-dependent manner.The ceils arrested at G2/M and S phases were markedly increased in Huaier and DDP combined treatment groups.The expression of CyclinB1 was down-regulated and expression of p-ATM,γ-H2AX was up-regulated in Huaier and/or DDP treatment groups.Conclusion Huaier and DDP can change cell cycle through up-regulation of p-ATM,γ-H2AX expression and down-regulation of CyclineB1 expression in human gastric cancer SGC7901 cells.关键词
胃癌/槐耳/DDP/细胞周期Key words
Gastric cancer/Huaier/DDP/Cell cycle引用本文复制引用
谢华夏,徐志远,郑国淀,黄挺,郑国威,滕飞,吕航,程向东..槐耳清膏联合顺铂改变胃癌SGC7901细胞周期分布的实验研究[J].浙江医学,2017,39(14):1155-1157,1174,4.基金项目
浙江省重大科技专项计划项目(2013C03044-4) （2013C03044-4）
