浙江医学2017，Vol.39Issue(14)：1175-1178,后插3,5.DOI:10.12056/j.issn.1006-2785.2017.39.14.2017-288
FPT Ⅲ对哮喘大鼠气道平滑肌细胞分泌IL-6及Rantes的影响
Effects of FPT Ⅲ on the secretion of IL-6 and Rantes in airway smooth muscle cells of asthmatic rats
摘要
Abstract
Objective to investigate the effects of FPTⅢ on the secretion of IL-6 and Rantes in airway smooth muscle cells of asthmatic rats.Methods To establish the rat model of asthma,the enzyme digestion method is adopted to cultivate airway smooth muscle cells,identified by immunohistochemistry and immunofluorescence.After using p21Ras specific inhibitor FPTⅢ intervention,IL-6 and Rantes were determinated by ELISA.The intracellular distribution of α-Actin was observed by fluorescence microscopy.Results The fluorescence intensity of α-Actin was stained by fluorescence,and the fluorescence intensity of the blank group was weaker than that of the control group.Secretion of IL-6 and Rantes were significantly decreased (P＜0.05) in asthmatic group after using FPT Ⅲ,and the inhibitory effect was time-dependent.When the concentration of FPT Ⅲ was up to 5-10 M,the inhibitory effect reached the maximum.Conclusion FPT Ⅲ intervention can make the asthmatic rat airway smooth muscle cells secreting IL-6 and Rantes decreased,accompanied by intracellular α-Actin changes,which further leads to the change of cell phenotypes,provide new ideas for the treatment of asthma.关键词
气道平滑肌细胞/FPT Ⅲ/哮喘Key words
Airway smooth muscle cells/FPT Ⅲ/Asthma引用本文复制引用
李娜,邱晨,荀安营,季乐财,卓宋明..FPT Ⅲ对哮喘大鼠气道平滑肌细胞分泌IL-6及Rantes的影响[J].浙江医学,2017,39(14):1175-1178,后插3,5.基金项目
深圳市龙岗区科技计划项目(201505143001004) （201505143001004）
