植物营养与肥料学报2017，Vol.23Issue(4)：914-923,10.DOI:10.11674/zwyf.16357
长期有机无机肥配施对东北黑土真菌群落结构的影响
Structure of soil fungal communities under long-term inorganic and organic fertilization in black soil of Northeast China
摘要
Abstract
[Objectives] The abundance,diversity and composition of fungal community were identified,and the main relationship between soil fungi community variation and soil fertilizer would be revealed in this paper.[Methods] Based on 36-years' fertilization experiment in Heilongjiang Academy of Agricultural Sciences,four treatments were selected to study:no fertilizer (CK);manure (M);chemical N,P and K fertilizer (NPK);and manure with NPK(MNPK).The ITS gene was applied as target gene,using Illumina Miseq sequencing and quantitative PCR method.The fungal abundance,diversity and composition in black soil were analyzed.The relationship of fertilization and fungi community was calculated.[Results] Long term application of chemical fertilizers reduced soil pH and led to soil acidification,while manure did not show same impact.ITS gene abundance in ratio in MNPK (26.91 × 104) was significantly higher than that in NPK,and the ratio was in order of MNPK ＞ M ＞ CK ＞ NPK.NPK treatment was more than in MNPK.Bacteria-to-fungi ratio was positively correlated with soil pH.Alpha diversity of fungi community of MNPK was slightly greater than that of NPK.Ascomycota and Zygomycota were the top two majority fungi found in black soil.The relative abundance of the fungi community was significantly different among the four fertilization treatments.The relative abundanceof Ascomycota in CK was 45.35％,in MNPK and NPK were 50.93％ and 56.16％,respectively.Manure reduced the relative abundance of pathogenic fungi.The relative abundances of highly aggressive Cochliobolus in MNPK (0.41％) and M (0.39％) were significantly lower than those of CK (3.25％) and NPK (2.08％).Canonical correlation analysis indicated that the selected soil properties could explain 73.3％ of the variation,and fungal communities were closely positively correlated with soil available P (Contribution =32.4％,P =0.002),NH4+-N concentration (Contribution =14.8,P =0.01) and NO3-N concentration (Contribution =16.2％,P =0.048).[Conclusions] Long-term fertilization affects fungal abundance,diversity and community characteristics of black soil.Compared with chemical fertilizers,manure alone and manure combined with chemical NPK fertilizers will improve the fungal communities structures,decrease fungal abundance and increase diversity activity,enhance the soil pH,slowed down soil acidification.Among the soil fertility factors,the soil available P,NH4+-N and NO3--N concentrations are the three dominant factors determining the variation of fungal communities structures.关键词
施肥/真菌群落/高通量测序/土壤有效磷/土壤铵态氮/土壤硝态氮Key words
fertilization/fungal communities/high-throughput sequencing/soil available P/soil NH4+-N/soil NO3--N引用本文复制引用
丁建莉,姜昕,马鸣超,关大伟,赵百锁,魏丹,曹凤明,李力,李俊..长期有机无机肥配施对东北黑土真菌群落结构的影响[J].植物营养与肥料学报,2017,23(4):914-923,10.基金项目
国家自然科学基金项目(41573066) （41573066）
国家“973”计划(2015CB150506) （2015CB150506）
农业微生物产品质量安全风险评估专项任务(GJFP201601202,GJFP201601203) （GJFP201601202,GJFP201601203）
国家“863”计划(2013AA102802-04) （2013AA102802-04）
国家现代农业产业技术体系建设专项(CARS-04)资助. （CARS-04）
