植物营养与肥料学报2017，Vol.23Issue(4)：973-982,10.DOI:10.11674/zwyf.16416
不同氮形态对镉胁迫下小白菜生长及镉含量的影响
Effects of nitrogen forms on the growth and Cd concentration of pakchoi (Brassica chinensis L.) under Cd stress
摘要
Abstract
[Objectives] Effects of common nitrogen (N) supply forms (ammonium,nitrate,ammonium/nitrate and urea) on the growth of Chinese cabbage pakchoi (Brassica chinensis L.) grown in the cadmium (Cd)-contaminated soil were studied to develop proper nitrogen management strategies for Cd-contaminated soils.[Methods] A pot experiment was conducted using soils from vegetable garden in Hangzhou.The tested soil was adjusted to four Cd contamination levels (0,1,3 and 5 mg/kg) with CdC12 solution in advance.Four N supply forms [ammonium,nitrate,ammonium/nitrate (1 ∶ 1) and urea] were supplied for the pakchoi respectively,with the addition amount of N 400 mg/kg soil.After the harvest,the biomass,photosynthesis,Cd-induced oxidative stress and Cd concentration of the plants in different treatments were investigated.[Results] 1) Compared with the Cd 0,the fresh weights of the edible parts ofpakchoi were reduced by 31％,16％,21％ and 26％ in Cd 1,by 58％,28％,35％ and 39％ in Cd 3,and by 83％,38％,52％ and 69％ in Cd 5 in the presence of ammonium,nitrate,ammonium/nitrate and urea,respectively.There were no significant differences in the tolerance index (TIcd) between the nitrate and ammonium/nitrate treatments,and the tolerance indices of the nitrate and ammonium/nitrate treatments were higher than those of the ammonium and urea treatments.2) Compared with Cd 0,the photosynthetic rates in the plants exposed to ammonium,nitrate,ammonium/nitrate,and urea were significantly decreased by 14％,10％,12％ and 13％ in Cd 1,by 33％,22％,25％ and 40％ in Cd 3,and by 53％,42％,41％ and 56％ in Cd 5;the levels of MDA and O2-production rates were increased by 11％,4％,9％ and 11％,and 5％,1％,2％ and 4％ in Cd 1,respectively.The Cd induced-photosynthesis inhibition and oxidative stress were relatively lower in the pakchoi with nitrate supply.3) The content of Cd in pakchoi were in the order of:nitrate ＜ ammonium/nitrate ＜ urea and ammonium treatments.[Conclusions] In the Cd-contaminated soils,nitrate N was more effective than ammoniam N in alleviating the Cd induced photosynthesis inhibition and oxidative stress and ultimately decreased the Cd contents in plants.关键词
镉/速效氮/小白菜/光合作用/氧化胁迫/镉含量Key words
cadmium/readily auailable nitrogen/pakchoi/photosynthesis/oxidative stress/cadmium content引用本文复制引用
潘维,徐茜茹,卢琪,刘越,薛琬蕾,宋必秀,都韶婷..不同氮形态对镉胁迫下小白菜生长及镉含量的影响[J].植物营养与肥料学报,2017,23(4):973-982,10.基金项目
浙江省公益技术应用研究项目(2017C32001) （2017C32001）
大学生科技创新项目(GJ201723003,2016r408057,CX201723005)资助. （GJ201723003,2016r408057,CX201723005）
