中国中西医结合杂志2017，Vol.37Issue(8)：950-954,5.DOI:10.7661/j.cjim.20170614.136
脾肾阳虚型肠易激综合征大鼠模型的建立与评价
Establishment and Evaluation of Pi-Shen Yang Deficiency Syndrome of Diarrhea-Predominant Irritable Bowel Syndrome Rat Model
摘要
Abstract
Objective To observe the establishment method for combination of disease and syndrome in Pi-Shen yang deficiency syndrome of diarrhea-predominant irritable bowel syndrome (IBS-D)rat model.Methods Totally 38 male SD rats,4 weeks old,were randomly divided into the normal group (n=10),the model group (n=14),and Wenshen Jianpi Recipe (WJR) group (n=14).The rat model was prepared by physicochemical stimulation,i.e.,a whole period (4 weeks) of senna gavage plus 2 weeks of acetic acid enema + fixation bound,2 weeks balloon rectal stimulation + rat tail clip.After modeling rats in the WJR group were administered with WJR by gastrogavage at the daily dose of 13.98 g/kg.Equal volume of distilled water was administered to rats in the model group.No intervention was given to rats in the normal group.Morphological manifestations and body weight were observed.The number of defecation,fecal water content,Bristol grade,intestinal sensitivity,intestinal motility were compared,and histopathological performance of intestinal mucosa observed.Results Compared with the normal group,rats in the model group were in poor mental state,liable to be irritated,with no obvious change in body weight increment (P＞0.05).They defecated more granules (P ＜0.05).The feces contained more water (P ＜0.05).Bristol type 5,6,7 increased more obviously.The intestinal sensitivity and motility increased (P ＜0.05).Compared with the model group,the motion was more active,with occasionally irritable or no obvious change in body weight in the WJR group (P＞0.05).They defecated more granules with less water content in feces (P＜0.05).Bristol type conversed to type 4.The intestinal sensitivity and motility gradually decreased (P＜0.05).There was no obvious abnormalities in intestinal mucosal histopathology of rats in each group.Conclusion This model method of physicochemical stimulation could be used as an ideal way for preparing IBS-D rats with Pi-Shen yang deficiency syndrome.关键词
脾肾阳虚证/腹泻型肠易激综合征/病证结合/动物模型Key words
Pi-Shen yang deficiency syndrome/diarrhea-predominant irritable bowel syndrome/combination of disease and syndrome/animal model引用本文复制引用
李依洁,魏玮,苏晓兰,杨晨,郭宇,朱佳杰,宋熠林,王贵澍,王庆国,陈建德..脾肾阳虚型肠易激综合征大鼠模型的建立与评价[J].中国中西医结合杂志,2017,37(8):950-954,5.基金项目
国家自然科学基金青年基金资助项目(No.81403389) （No.81403389）
