中国中西医结合杂志2017，Vol.37Issue(8)：979-984,6.DOI:10.7661/j.cjim.20170703.146
平冲降逆方对裸鼠人子宫膜异位症模型凋亡及TNF-α、IL-8表达的影响
Effect of Pingchong Jiangni Recipe on Apoptosis and Expressions of TNF-α and IL-8 in Nude Endometriosis Model Mice
摘要
Abstract
Objective To observe the effects of Pingchong Jiangni Recipe (PCJNR)on apoptosis and the expressions of TNF-α and IL-8 in nude mice of Endometriosis model.Methods A total of 48 BALB/c-nu female nude mice were randomly divided into two groups,EMs-derived model group and nonEMs-derived model group,24 in each group.Mice in EMs-derived model group were implanted with the endometrium from EMs patients,while those in non-EMs group were implanted with the endometrium from patients without EMs.Then the two groups were randomly subdivided into three groups respectively,traditional Chinese medicine (TCM) group,positive drug group and negative control group,8 mice in each group.They were respectively administrated with PCJNR,mifepristone,and equal volume of physiological saline for 4 weeks.Histological morphology and ultrastructure of implanted foci,cell apoptosis,and expressions of TNF-α and IL-8 were observed.Results The volume of foci were more shrunk and the geneses of peripheral vessels were less in the TCM group than in the negative control group,no matter with EMs or without EMs.Degeneration of tunica intima and apoptosis could also be seen under light microscope and electron microscope.Results of immunohistochemical assay showed that the apoptotic rate was significantly higher in the TCM group than in corresponding negative control groups,but the expressions of TNF-α and IL-8 were significantly lower in the TCM group than in corresponding negative control groups.Conclusion PCJNR significantly inhibited the growth of ectopic endometriosis in nude mice,and the effect might be achieved by inducing cell apoptosis and down-regulating expressions of TNF-α and IL-8.关键词
子宫内膜异位症/平冲降逆方/化瘀通络/凋亡/TNF-α/IL-8Key words
endometriosis/Pingchong Jiangni Recipe/removing stasis and dredging collaterals/apoptosis/TNF-α/IL-8引用本文复制引用
梁瑞宁,施旻,占诗艳,陈振,李佩双,孙雪燕..平冲降逆方对裸鼠人子宫膜异位症模型凋亡及TNF-α、IL-8表达的影响[J].中国中西医结合杂志,2017,37(8):979-984,6.基金项目
国家自然科学基金资助项目(No.81160447) （No.81160447）
江西省卫生厅资助项目(No.2013A172) （No.2013A172）
