中国中西医结合杂志2017，Vol.37Issue(8)：985-990,6.DOI:10.7661/j.cjim.20170703.148
补肾活血方对血管性痴呆大鼠海马BDNF mRNA及受体TrkB m RNA表达的影响
Effect of Bushen Huoxue Recipe on mRNA Expression of BDNF and Its Receptor TrkB in Hippocampus of Vascular Dementia Rats
摘要
Abstract
Objective To observe the protective mechanisms of Bushen Huoxue Recipe (BHR)on hippocampal neurons in vascular dementia (VD) rats.Methods Totally 70 SD rats were randomly divided into 5 groups,i.e.,the sham-operation group,the model group,high and low dose BHR groups,and the nimodipine group,14 in each group.The VD rat model was prepared by blocking two blood vessels.Rats in the high dose BHR group were administered with BHR (10.14 g crude drugs/kg) by gastrogavage.Rats in the low dose BHR group were administered with BHR (5.07 g crude drugs/kg) by gastrogavage.Rats in the nimodipine group were administered with Nimodiping solution (11.06 mg/kg) by gastrogavage.Thirty days after administration,praxiological changes were detected by water maze.Ultramicrostructure of hippocampal neuron,gene and protein expression of BDNF mRNA and TrkB mRNA were also observed.Results Compared with the sham-operation group,the ability of learning and memory exploration were reduced in VD rats of the model group,manifested as escape latency period obviously extended,times for passing platform and detention time in platform obviously decreased (P ＜0.01),levels of mRNA and protein expressions of BDNF and TrKB in hippocampal neuron showing decreasing tendency (P ＜0.05,P ＜0.01),and the ultramicrostructure of hippocampal neuron obviously damaged.Compared with the model group,the ability of learning and memory exploration were improved in high and low dose BHR groups,manifested as escape latency period obviously shortened,times for passing platform and detention time in platform obviously inecreased (P ＜0.05,P ＜0.01),levels of mRNA and protein expressions of BDNF and TrKB in hippocampal neuron elevated (P ＜0.05,P ＜0.01),and the ultramicrostructure of hippocampal neuron obviously improved.Conclusion BHR could improve the expression levels of BDNF and its receptor TrkB,and protect hippocampal neuron,so as to improve the learning and memory ability of VD rats.关键词
补肾活血方/血管性痴呆/脑源性神经生长因子/酪氨酸蛋白激酶B/超微结构Key words
Bushen Huoxue Recipe/vascular dementia/brain-derived neurotrophic factor/tyrosine protein kinase B/ultramicrostructure引用本文复制引用
于文涛,高维娟,方朝义,张一昕,吴中秋..补肾活血方对血管性痴呆大鼠海马BDNF mRNA及受体TrkB m RNA表达的影响[J].中国中西医结合杂志,2017,37(8):985-990,6.基金项目
河北省自然科学基金资助项目(No.H2015423057) （No.H2015423057）
