动物营养学报2017，Vol.29Issue(3)：898-905,8.DOI:10.3969/j.issn.1006-267x.2017.03.021
不同来源木聚糖酶及其组合对木聚糖水解产物组成、细菌增殖与大肠杆菌黏附性的影响
Effects of Different Sources of Xylanase and Their Combinations on Xylan Hydrolysate Composition, Bacteria Proliferation and Escherichia coli Adhesion
摘要
Abstract
This experiment was conducted to study the hydrolysis effect of different sources of xylanase and their combinations on xylan,and then research the effects of xylan hydrolysate on bacteria proliferation and adhesion of Escherichia coli to intestinal epithelial cells (IEC).Xylanases from Pichia pastoris and Aspergillus oryzae were named xylanase A and xylanase B,respectively.The xylanase A,xylanase B,combined enzyme 1 (xylanase A:xylanase B =3:7),combined enzyme 2 (xylanase A:xylanase B =1∶1),combined enzyme 3 (xylanase A ∶xylanase B =7∶3) were used to hydrolyze xylan,respectively.The effects of xylan hydrolysate on Escherichia coli,Bacillus subtilis and Lactobacilli proliferation and the adhesion of Escherichia coli to IEC were measured.The results showed as follows:1) some combined effects of two kinds of xylanase were observed.The total content of xylobiose and xylotriose in xylanase A,xylanase B,combined enzyme 1,combined enzyme 2 and combined enzyme 3 groups were 95.70％,86.79％,93.11％,94.55％ and 87.55％,respectively.The content of xylobiose in xylanse A group was the highest,while the highest content of xylotriose in combined enzyme 2 group.2)Five kinds of xylan hydrolysate had no significant effect on the proliferation [expressed in optical density (OD) value] of Escherichia coli cultured at 20 hours (P＞0.05),but significantly improved the proliferation of Bacillus subtilis cultured at 24 and 30 hours (P＜0.05),and significantly improved the proliferation of Lactobacilli cultured at 13 and 17 hours (P＜0.05).3) Five kinds of xylan hydrolysate could significantly reduced the adhesion rate of Escherichia coli to IEC(P＜0.05).It is concluded that xylan hydrolyzed by difference sources of xylanase and their combinations can produce xylan hydrolysate with xylobiose and xylotriose as main components,which can promote Bacillus subtilis and Lactobacillus proliferation and reduce the adhesion of Escherichia coli to IEC.关键词
木聚糖酶/木聚糖水解产物/细菌增殖/肠道上皮细胞/细菌黏附率Key words
xylanase/xylan hydrolysate/bacteria proliferation/IEC/bacteria adhesion rate分类
农业科技引用本文复制引用
欧阳海燕,雷钊,马岩,冯定远,张莹,叶慧,夏旻灏,曹庆云,左建军..不同来源木聚糖酶及其组合对木聚糖水解产物组成、细菌增殖与大肠杆菌黏附性的影响[J].动物营养学报,2017,29(3):898-905,8.基金项目
广东省科技计划项目(2016A020210104) （2016A020210104）
广州市科技计划项目(201510010258) （201510010258）
