动物营养学报2017,Vol.29Issue(3):970-978,9.DOI:10.3969/j.issn.1006-267x.2017.03.030
产几丁质酶菌株的筛选、鉴定及其对玉米秸秆中优势霉菌的抑制作用
Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk
摘要
Abstract
The objective of this experiment was to inhibit the growth of dominant moulds in corn stalk using chitinase producing strain,and provide a reference for improving the utilization efficiency of corn stalk.Chitinase producing strain of BS-1 was screened from corn stalk using colloidal chitin medium.At the same time,the dominant moulds were isolated and purified.Identification of strain BS-1 and dominant moulds were carried out through morphological observation and 16S rDNA or 18S rDNA sequence analysis.In addition,the chitinase activity of strain BS-1 was assayed using 3,5-dinitrosalicylic acid colorimetric method after 24,48,72,96,120,144 and 168 h of fermentation and the inhibition action of fermentation liquid of strain BS-1 to dominant moulds was detected by oxford cup method.The results showed as follows:strain BS-1 isolated from corn stalk was Bacillus subtilis and four dominant moulds were Mucor circinelloides,Fusarium oxysporum,Aspergillus oryzae and Aspergillus niger,respectively.After 120 h of fermentation at 37 ℃,the highest chitinase activity of strain BS-1 was obtained to be 3.23 U/mL.The fermentation liquid of strain BS-1 showed obvious inhibition to four dominant moulds from corn stalk,the diameter of inhibition zone was 18.13,18.48,17.55 and 15.68 mm,respectively.In conclusion,the screened chitinase producing Bacillus subtilis BS-1 can effectively inhibit the growth of dominant moulds of Mucor circinelloides,Fusarium oxysporum,Aspergillus oryzae and Aspergillus niger from corn straw.关键词
玉米秸秆/优势霉菌/抑菌/几丁质酶/枯草芽孢杆菌Key words
corn stalk/dominant moulds/antifungal/chitinase/Bacillus subtilis分类
农业科技引用本文复制引用
张立阳,张幸怡,张薇,林聪,张永根..产几丁质酶菌株的筛选、鉴定及其对玉米秸秆中优势霉菌的抑制作用[J].动物营养学报,2017,29(3):970-978,9.基金项目
国家奶牛产业技术体系项目(CARS-37) (CARS-37)
寒区规模化奶牛场奶牛精细饲养与粗饲料高效利用关键技术集成与示范(2012BAD12B05-1) (2012BAD12B05-1)