动物营养学报2017，Vol.29Issue(3)：1039-1045,7.DOI:10.3969/j.issn.1006-267x.2017.03.038
茶皂素对奶牛免疫功能的影响
Effects of Tea Saponin on Immune Function of Dairy Cows
摘要
Abstract
This experiment was conducted to study the effects of tea saponin on serum immune related cytokines contents and their mRNA expression levels of Holstein cows.Four rumen fistulated cows were used following a 4×4 Latin square design with different infusion concentrations of tea saponin [0 (control),15,30,45 g/d].The experiment had 4 periods,and each period consisted of 14 d per feeding period and 7 d sampling period.Enzyme-linked immunosorbent assay (ELISA) method kit was used to measure the contents of immunoglobulins,interleukin and tumor necrosis factor alpha (TNF-α) in serum.Quantitative real-time PCR was used to measure the mRNA expression levels of interleukin-1 (IL-1),interleukin-6 (IL-6),interleukin-10 (IL-10) and TNF-α.The results showed as follows:1) the serum immunoglobulin M (IgM) content of dairy cow in 30 g/d tea saponin group was significantly higher than that in control group (P＜0.05).2) The serum IL-1 content in 15 g/d tea saponin group was significantly higher than that in control group and 45 g/d tea saponin group (P＜0.05).3) Compared with the control group,the mRNA expression levels of IL-1,IL-10,IL-6 and TNF-α in 15,30 and 45 g/d tea saponin groups were not significantly different (P＞0.05),but the mRNA expression levels of above cytokines in 45 g/d tea saponin group was increased.In conclusion,tea saponin can improve the contents of immunoglobulins and immune related cytokines in serum,as well as the mRNA expression level of cytokine IL-6,which indicate that tea saponin can improve the immune function of dairy cows.关键词
茶皂素/免疫/细胞因子/奶牛Key words
tea saponin/immunity/cytokines/dairy cows分类
农业科技引用本文复制引用
常肖肖,张议夫,赵士萍,蒋琦辉,王炳,方洛云,熊本海,蒋林树..茶皂素对奶牛免疫功能的影响[J].动物营养学报,2017,29(3):1039-1045,7.基金项目
“十二五”国家科技支撑项目(25012BAD14B09) （25012BAD14B09）
“十三五”国家重大科技专项(2016YFDO70020,2016YFDO700205) （2016YFDO70020,2016YFDO700205）
北京市农业局北京市现代农业产业技术体系奶牛创新团队项目 （）
