高等学校化学学报2017，Vol.38Issue(8)：1368-1374,7.DOI:10.7503/cjcu20170127
利用活体成像技术研究海茸β-1,3/1,6-葡聚糖在小鼠体内的分布
Detection of Animal Tissue Distribution of β-1,3/1,6-Glucan from Durvillaea antarctica by in vivo Imaging
摘要
Abstract
In the present study, β-1,3/1,6-glucan(DAG) from Durvillaea antarctica was investigated.First, the functional amino group was introduced into the DAG reduction site by reductive amination reaction.The reaction product was then coupled with NHS-activated fluorescent dye(Cy7), and the Cy7-labeled DAG molecule was obtained subsequently.The distribution of DAG in mice was studied by small animal in vivo imaging technique.The results show that DAG is mainly distributed in the lungs, livers and kidneys, which possessed the characteristics of targeting the mouse lungs and excreting through the kidneys.The method is simple and reliable, which provides an important reference for the distribution and metabolism study of polysaccharides and oligosaccharides in vivo.关键词
β-1,3/1,6-葡聚糖/荧光标记/活体成像/组织分布Key words
β-1/3/1/6-Glucan/Fluorescence labeling/In vivo imaging/Tissue distribution分类
化学化工引用本文复制引用
赵小亮,刘曦,杨忆,蔡超,吕友晶,李国云,郝杰杰,于广利..利用活体成像技术研究海茸β-1,3/1,6-葡聚糖在小鼠体内的分布[J].高等学校化学学报,2017,38(8):1368-1374,7.基金项目
国家自然科学基金(批准号: 31670811), 山东省科技重大专项(批准号: 2015ZDJS04002)和泰山学者专项经费资助.Supported by the National Natural Science Foundation of China(No.31670811), the Major Projects of Science and Technology in Shandong Province, China(No.2015ZDJS04002) and the Program for Taishan Scholar of Shandong Province, China. （批准号: 31670811）
