国际病理科学与临床杂志2017，Vol.37Issue(8)：1560-1564,5.DOI:10.3978/j.issn.2095-6959.2017.08.002
急性肺损伤小鼠肺内热休克蛋白A12B的表达
Expression of HSPA12B in lung of acute lung injury mice
摘要
Abstract
Objective: To observe the expression of heat shock protein A12B (HSPA12B) in lungs of acute lung injury (ALI) mice and the effect of lipopolysaccharide (LPS) on the expression of HSPA12B in vascular endothelial cells. Methods: Mouse was injected with LPS (5 mg/kg), and lung tissue was taken at 6 h. The expression of HSPA12B mRNA and protein in lung tissue was detected by real-time PCR and Western blot. In vitro, human umbilical vein endothelial cells were stimulated by LPS (1 μg/mL). Real-time PCR and Western blot were used to detect the expression of HSPA12B mRNA and protein. NF-κB signaling pathway inhibitor PDTC was used to observe the possible molecular mechanisms of LPS-induced HSPA12B expression. Results: Compared with the normal group, the expression of HSPA12B mRNA and protein in LPS-induced lung tissue of ALI mice were significantly increased. The expression of HSPA12B mRNA and protein in human umbilical vein endothelial cells were up-regulated in a time-dependent manner. NF-κB inhibitor (PDTC) pretreatment reversed the LPS-induced HSPA12B mRNA and protein upregulation. Conclusion: The expression of HSPA12B in lung of ALI mice increases, and its mechanism is related to the activation of NF-κB signaling pathway.关键词
急性肺损伤/热休克蛋白A12B/脂多糖/脐静脉内皮细胞/小鼠Key words
acute lung injury/heat shock protein A12B/lipopolysaccharide/umbilical vein endothelial cells/mouse引用本文复制引用
董良,刘富兵,陈永琴..急性肺损伤小鼠肺内热休克蛋白A12B的表达[J].国际病理科学与临床杂志,2017,37(8):1560-1564,5.基金项目
遵义医学院附属医院博士启动基金 (201703) （201703）
遵义医学院大学生创新创业训练计划项目 (20162312).This work was supported by Scientific Research Foundation for Doctor of Zunyi Medical Col ege (201703) （20162312）
Student's Platform for Innovation and Entrepreneurship Training Program of Zunyi Medical Col ege (20162312), China. （20162312）
