临床与病理杂志2017，Vol.37Issue(8)：1571-1575,5.DOI:10.3978/j.issn.2095-6959.2017.08.004
胡椒碱对卵巢癌细胞株SKOV-3侵袭与凋亡的影响及其机制
Effect and mechanism of piperine on invasion and apoptosis of SKOV-3 ovarian cancer cell line
许静 1郭哲 1孙慧霞 1梁殿迅1
作者信息
- 1. 南阳市中心医院妇产科,河南 南阳 473000
- 折叠
摘要
Abstract
Objective: To investigate the effects of piperine on SKOV-3 ovarian cancer cell line invasion and apoptosis and to explore the underlying mechanisms. Methods: SKOV-3 cells were treated with different concentrations of piperine (0, 5, 10 or 20 μmol/L) for different times (12, 24 or 36 h), cell viability was assessed by CCK-8 assay. The invasion and apoptosis of SKOV-3 cells were determined with Transwell and TUNEL staining, respectively. The protein expression of p-PI3K and p-Akt were examined by Western blot. Results: CCK-8 results showed that piperine decreased the viability of SKOV-3 cells in a dose-dependent and time-dependent manner. After treatment with 10 μmol/L of piperine for 36 h, cell invasion was significantly inhibited, whereas the apoptotic cell number was markedly increased. Moreover, the protein expression of p-PI3K and p-Akt were significantly decreased after piperine treatment. Conclusion: Piperine can inhibit SKOV-3 cell viability and invasion, and increase cell apoptosis, which may through inhibition of PI3K/Akt signaling pathway.关键词
胡椒碱/卵巢癌细胞/PI3K/Akt信号通路Key words
piperine/ovarian cancer cells/PI3K/Akt signaling pathway引用本文复制引用
许静,郭哲,孙慧霞,梁殿迅..胡椒碱对卵巢癌细胞株SKOV-3侵袭与凋亡的影响及其机制[J].临床与病理杂志,2017,37(8):1571-1575,5.
