国际病理科学与临床杂志2017，Vol.37Issue(8)：1576-1582,7.DOI:10.3978/j.issn.2095-6959.2017.08.005
TCF21对喉癌Hep-2细胞生物学行为的影响
Effect of TCF21 on cellular biological characteristics in laryngeal carcinoma Hep-2 cell
杨庆军1
作者信息
- 1. 周口市中心医院耳鼻咽喉头颈外科,河南 周口 466000
- 折叠
摘要
Abstract
Objective: To investigate the effect and mechanism of transcription factor 21 (TCF21) on the cellular biological characteristics in laryngeal carcinoma cell line Hep-2. Methods: Lentiviral transfection was used to up-regulated TCF21 expression in Hep-2 cells, and then cell proliferation and apoptosis were detected with Cell Counting Kit-8 (CCK-8) assay and flow cytometry assay, respectively. The expression of cell cycle- and apoptosis-related molecules at protein level was measured by Western blot. Results: Over-expression of TCF21 suppressed cell proliferation and cell cycle, while promoted cell apoptosis in Hep-2 cell (P<0.05). Western blot showed that the expression of cyclinD1, CDK4, CDK6, p-Rb and Bcl-2 at protein level decreased significantly after up-regulation of TCF21, with an increased expression of P21, Bax and cleaved-caspase-3 (P<0.05). Conclusion: In Hep-2 cells, over-expression of TCF21 could suppress cell proliferation and cell cycle, while promote cell apoptosis via regulating the expression of cell cycle- and apoptosis-related molecules, suggesting that TCF21 may be a potential new target for diagnosis and targeted therapy of laryngeal carcinoma.关键词
转录因子21/喉癌/增殖/凋亡Key words
transcription factor 21/laryngeal carcinoma/proliferation/apoptosis引用本文复制引用
杨庆军..TCF21对喉癌Hep-2细胞生物学行为的影响[J].国际病理科学与临床杂志,2017,37(8):1576-1582,7.
