广西师范大学学报（自然科学版）2017，Vol.35Issue(3)：133-140,8.DOI:10.16088/j.issn.1001-6600.2017.03.017
基于线粒体16S rDNA基因序列沼虾属的系统发育研究
Phylogeny of Macrobrachium Species Using Mitochondrial 16S Ribosomal DNA
摘要
Abstract
To understand the evolutionary relationship among the Macrobrachiumn species,mitochondrial partial 16S ribosomal DNA of M.rosenbergii was amplified and sequenced.By sequence alignment from 511 characters,178 characters are variable and 162 characters are parsimony informative.The parameters specified under optimum model were nucleotide frequencies of ηA =0.306 3,ηc =0.065 7,ηG=0.204 6,ηT=0.423 3;reversal rates of change for [A-C]=0.042 7,[A-G]=0.248 59,[A-T]=0.028 4,[C-G]=0.032 9,[C-T]=0.629 0,[G-T]=0.018 4.The proportion of invariant sites was 0.428 7,and gamma shape distribution a =0.429 8.No substitution saturation was found as the level of transitional base substitutions was higher than the level of transversions over all genetic distances.Bayes phylogenetic tree was constructed by using Bayesian method.The out group Palaemon formed as paraphyletic to Macrobrachium with significant bootstrap value.Principally all species clustered into 3 big clades as their evolutionary relationship,in which,M.malayanum constitute a big clade,M.carcinus,M.americanum and M.vollenhovenii constituted another big clade,and the other 32 Macrobrachium constituted the third big clade.M.rosenbergii,M.japonicum,M.meridionalis,M.gracilirostre,M.jaroense,M.horstii,M.placidulum,M.lepidactyloides,M.lar,M.latimanus and M.ohione formed one cluster.关键词
沼虾属/线粒体DNA/16S rDNA/系统发生Key words
Macrobrachium/mitochondrial DNA/16S rDNA/molecular phylogeny分类
农业科技引用本文复制引用
张小敏,林勇,宾石玉,余艳玲,曾兰,钟丹丹,张永德..基于线粒体16S rDNA基因序列沼虾属的系统发育研究[J].广西师范大学学报（自然科学版）,2017,35(3):133-140,8.基金项目
广西科学研究与技术开发计划项目(桂科能14121008-4-1) （桂科能14121008-4-1）
国家现代农业产业技术体系广西罗非鱼创新团队建设项目(nycytxgxcxtd-08-01) （nycytxgxcxtd-08-01）
