河南农业科学2017，Vol.46Issue(7)：9-17,85,10.DOI:10.15933/j.cnki.1004-3268.2017.07.002
利用重组自交系群体定位不同温度条件下玉米种子发芽性状的QTL
QTL Mapping of Seed Germination Traits under Different Temperature Conditions Using Recombinant Inbred Lines of Maize
摘要
Abstract
Two recombinant inbred lines (RILs) populations which were obtained from Yu 537A × Shen 137 and Yu 82 × Shen 137 and contained 420 family lines were used as materials,two high-density molecular linkage maps were constructed using single nucleotide polymorphism (SNP) molecular marker technology.Through composite interval mapping method,the QTLs of seed vigor-related morphological and physiological traits were detected,including percentage of germination (GP),germination energy (GE),germination index (GI),vigor index (VI),seedling length (SL),seedling dry weight (SDW),root dry weight(RDW).Sixty-four QTLs for seed vigor-related traits were detected in the two connected RILs population in normal and low temperature conditions.The results showed that twenty-four QTLs were found in the population based on Yu 82 × Shen 137,and were located on chromosomes 1,2,3,4,5,6,7 and 10,with the explanation of phenotypic variation of a single QTL from 5.61％ to 11.01％.Others fourty QTLs were found in the population based on Yu 537A × Shen 137,and located on all chromosomes except 2,with the explanation of phenotypic variation of a single QTL from 5.39％ to 11.92％.Six mQTLs were identified on chromosomes 3,5,7 and 10 by meta-analysis,and twenty-five initial QTLs were integrated,the consolidation ratio was 39.1％.Each mQTL contained 4.17 QTLs on average,involved in the regulation of two to four traits,including VI,SDW,RDW,GE,mQTL3-2 contained 7 QTLs.Thirty-five candidate genes were located in 6 mQTLs corresponding SNP marker distribution ranges,which involved in the regulation of seed germination,plant development,growth metabolic pathways,signal transduction,withstanding adversity and so forth.关键词
玉米/重组自交系/温度/发芽性状/QTL定位Key words
maize/recombinant inbred lines/temperature/germination trait/QTL mapping分类
农业科技引用本文复制引用
韩赞平,陈彦惠,刘海英,赵瑞芳,郭书磊..利用重组自交系群体定位不同温度条件下玉米种子发芽性状的QTL[J].河南农业科学,2017,46(7):9-17,85,10.基金项目
国家自然科学基金项目(U1504315) （U1504315）
河南科技大学青年基金项目(2015QN031) （2015QN031）
河南科技大学科研启动基金项目(13480067) （13480067）
