林产化学与工业2017，Vol.37Issue(3)：73-81,9.DOI:10.3969/j.issn.0253-2417.2017.03.010
大血藤原花色素抗氧化活性及酶抑制活性研究
Antioxidant Activity and Enzyme Inhibitory Activity of Proanthocyanidins from Sargentodoxa cuneata(Oliv) Rehd.et Wils.
摘要
Abstract
Proanthocyanidins were extracted from Sargentodoxa cuneata (Oliv) Rehd.et Wils.,and the crude extract was purified and isolated.The content of proanthocyanidins was determined by an improved vanillin-HCl colorimetric method.Meanwhile,the antioxidant activity and enzyme inhibitory activity of each sample were measured.The results showed that the contents of proanthocyanidins in the crude extract(CE),petroleum ether fraction(PE),ethyl acetate insoluble fraction(EIF),ethyl acetate fraction (EF),n-butanol fraction (BF) and water residue (WR) were (36.03 ± 0.40) ％,(1.19 ± 0.11) ％,(57.99±0.22)％,(32.40 ±0.07)％,(25.03 ±0.08)％ and (41.32 ±0.37)％,respectively.Moreover,the proanthocyanidin content of the sample purified by Sephadex LH-20 was (98.35 ± 0.72) ％,which was 2.73 times of that of the crude extract.The DPPH free radical scavenging activity of the crude extract(IC50 =178.10 ±2.67 mg/L) was 1.12 times of that of L-ascorbic acid(IC50 =78.89 ± 1.67 mg/L);the α-glucosidase inhibitory activity of purified sample was 120.71 times of that of acarbose and the corresponding acetylcholine inhibitory activity was 1.14 times of that of tacrine.It showed that there were abundant proanthocyanidins in S.cuneata and the proanthocyanidins had an obvious antioxidant activity and enzyme inhibitory activity in vitro.关键词
原花色素/DPPH自由基清除活性/糖苷酶抑制活性/胆碱酯酶抑制活性Key words
proanthocyanidins/DPPH free radical scavenging activity/glycosidase inhibitory activity/cholinesterase inhibitory activity分类
化学化工引用本文复制引用
李彦,李鑫,刘景玲,邓凯航,梁宗锁..大血藤原花色素抗氧化活性及酶抑制活性研究[J].林产化学与工业,2017,37(3):73-81,9.基金项目
"十二五"国家科技支撑计划项目(2015BAC01B03) （2015BAC01B03）
陕西省科技攻关计划(2015SF243) （2015SF243）
