南京医科大学学报（自然科学版）2017，Vol.37Issue(7)：830-835,6.DOI:10.7655/NYDXBNS20170709
microRNA-10b与胰腺癌吉西他滨耐药的相关性及机制研究
Relationship between microRNA-10b and chemoresistance of pancreatic cancer to gemcitabine and its possible mechanism
摘要
Abstract
Objectives:To reveal the relationship between microRNA-10b and gemcitabine (GEM) resistance of pancreatic cancer and its possible mechanism.Methods:The expression of microRNA-10b in PANC-1 cells (which are relatively resistant to GEM) and CFPAC-1 cells (which are relatively sensitive to GEM) were detected by RT-qPCR.PANC-1 and CFPAC-1 cells were treated with different doses of GEM,and then the cells were harvested.The expression of microRNA-10b was detected by RT-qPCR.CCK-8 assay and flow cytometry were performed to evaluate the drug sensitivity of CFPAC-1 cells,which were transfected with microRNA-10b mimics to GEM.The protein expressions of PI3K,p-Akt,Bcl-2 and Survivin in CFPAC-1 cells transfected with microRNA-10b mimics or negative control were demonstrated using Western blotting assay.The gene expressions of PTEN in CFPAC-1 cells transfected with microRNA-10b mimics or negative control were detected by RT-qPCR.Results:PANC-1 cells showed significantly increased expression of microRNA-10b as compared to CFPAC-1 cells.The expression of microRNA-10b both in PANC-1 and CFPAC-1 cells increased with the rising of GEM concentration.The sensitivity of CFPAC-1 cells to GEM decreased when microRNA-10b was overexpressed and the expressions of PI3K,p-Akt,Bcl-2,and Survivin were much higher than their control groups.The expression of PTEN mRNA decreased as microRNA-10b increased.Conclusions:MicroRNA-10b might negatively regulate the gene expression of PTEN,which increases the protein expressions of PI3K,p-Akt,Bcl-2 and Survivin,thus reduces the sensitivity of CFPAC-1 cells to GEM,and as a result,pancreatic cancer cells become resistant to GEM.关键词
microRNA-10b/胰腺癌/吉西他滨/化疗耐药Key words
microRNA-10b/pancreatic cancer/gemcitabine/chemoresistance分类
医药卫生引用本文复制引用
顾燊,邵欣宇,邹晓平..microRNA-10b与胰腺癌吉西他滨耐药的相关性及机制研究[J].南京医科大学学报（自然科学版）,2017,37(7):830-835,6.基金项目
江苏省医学领军人才与创新团队(LJ201104) （LJ201104）
