摘要
Abstract
Objective To investigate the effect of garlicin on vulnerable plaques in apolipoprotein E gene knockout (apoE-/-) mice with atherosclerosis and possible mechanisms.Methods The apoE-/ mice were given high-fat feed for 8 weeks to establish a mouse model of atherosclerosis,and then these mice were randomly divided into model group,low dose garlicin group,and high dose garlicin group,with 8 mice in each group.The mice in the model group were given 0.50 mL normal saline by gavage,those in the low-dose garlicin group were given 0.25 mL normal saline and 0.25 mL garlicin solution by gavage,and those in the high-dose garlicin group were given 0.50 mL garlicin solution by gavage once a day for 8 consecutive weeks.TUNEL staining was used to observe the area of plaque apoptosis in the aortic root,and immunohistochemistry was used to observe the numbers of apoptotic macrophages and vascular smooth muscle cells and the expression of apoptosis-related protein P53.Results Both low-and high-dose garlicin groups had a reduction in the area of plaque apoptosis after garlicin treatment by gavage;the lowdose garlicin group had a significant reduction (F=42.32,P＜0.05),while the high-dose garlicin group had no significant reduction (P＞0.05).Compared with the model group and the high-dose garlicin group,the low-dose garlicin group had a significant reduction in the number of apoptotic macrophages in the plaque (F =28.13,P ＜0.05);there was no significant difference in the number of apoptotic vascular smooth muscle cells in the plaque between groups (P＞0.05).The low-and high-dose garlicin groups had significantly lower expression of P53 in the plaque than the model group (F=22.11,P＜0.05).Conclusion Garlicin can stabilize the advanced unstable plaques by downregulating P53 expression and inhibiting cell apoptosis.关键词
斑块,动脉粥样硬化/大蒜素/载脂蛋白E类/小鼠,基因敲除/基因,p53Key words
plaque, atherosclerotic/garlicin/apolipoproteins E/mice, knockout/genes, P53分类
医药卫生
