青岛大学医学院学报2017，Vol.53Issue(3)：283-286,4.DOI:10.13361/j.qdyxy.201703009
灯盏生脉胶囊对帕金森病大鼠黑质区多巴胺能神经元损伤影响
EFFECT OF DENGZHAN SHENGMAI CAPSULES ON DOPAMINERGIC NEURON INJURY IN SUBSTANTIA NIGRA IN RATS WITH PARKINSON'S DISEASE
摘要
Abstract
Objective To investigate the effect of Dengzhan Shengmai capsules on dopaminergic neuron injury in the substantia nigra in rats with Parkinson's disease (PD) induced by 6-hydroxydopamine (6OHDA),as well as its mechanism in DNA oxidative damage repair.Methods A total of 80 rats were randomly divided into sham-operated group,model group,low-dose Dengzhan Shengmai group (low-dose group),and high-dose Dengzhan Shengmai group (high-dose group).Injection of 6-OHDA into the striatum was performed to establish a rat model of PD.The rats in the low-and high-dose Dengzhan Shengmai groups were given low-or high-dose Dengzhan Shengmai capsules by gavage from 3 days before modeling to week 5 before sacrifice,and those in the sham-operation group and the model group were given normal saline at the same dose.The changes in rotational behaviors induced by apomorphine were observed at week 4 after modeling.After the rats were beheaded and brain tissue was collected at week 5,immunohistochemistry and Western blot were used to measure the number of cells with tyrosine hydroxylase (TH) or MTH1 in the substantia nigra and their protein expression levels.Results There was a significant difference in the rotation rate between the four groups (x2 =21.89,P＜0.05).The number of cells with TH in substantia nigra pars compacta at the operation side was 78±13 in the sham-operation group,27±7 in the model group,39±8 in the low-dose group,and 54±11 in the high-dose group;there was a significant difference between the four groups (F =43.89,P ＜0.95).The high-dose group had a significantly higher content of TH protein in the substantia nigra at the operation side than the model group (F =9.50,P ＜0.05).The number of cells with MTH1 in the substantia nigra pars compacta at the operation side was 42±7 in the sham-operation group,22±6 in the model group,36±6 in the low-dose group,and 45±8 in the high-dose group;there was a significant difference between the four groups (F =22.65,P ＜0.05).The high-dose group had the highest MTH1 content in the substantia nigra at the operation side,while the sham-operation group had the lowest content,and there was a significant difference between these two groups (F =50.49,P ＜0.05).Conclusion Dengzhan Shengmai capsules can effectively alleviate dopaminergic neuron injury in the substantia nigra in rats with PD,and its mechanism is associated with the improvement in DNA damage repair.关键词
灯盏生脉胶囊/帕金森病/黑质/氧化嘌呤核苷磷酸酶/酪氨酸羟化酶Key words
Dengzhan Shengmai capsule/Parkinson's disease/substantia nigra/oxidized purine nucleoside triphosphatase/tyrosine hydroxylase分类
医药卫生引用本文复制引用
朱蔚文,孙卫文,王卫钢,梁采宇,李嘉滢..灯盏生脉胶囊对帕金森病大鼠黑质区多巴胺能神经元损伤影响[J].青岛大学医学院学报,2017,53(3):283-286,4.基金项目
广东省中医药局科研资助项目(20142095),广州医科大学校级科研项目(2015B017),2016年度广东省大学生创新创业训练计划项目(201610570036) （20142095）
