青岛大学医学院学报2017，Vol.53Issue(3)：297-299,304,4.DOI:10.13361/j.qdyxy.201703013
靶向抑制miR-106b对体外膀胱癌细胞增殖和凋亡影响
EFFECT OF TARGETED INHIBITION OF MICRORNA-106B ON PROLIFERATION AND APOPTOSIS OF IN VITRO BLADDER CANCER CELLS
摘要
Abstract
Objective To investigate the effect of targeted inhibition of microRNA-106b (miR-106b) on the proliferation,invasion,and apoptosis of in vitro bladder cancer cells.Methods Cell transfection was used to construct the colonies of bladder cancer cells with targeted inhibition of miR-106b,and quantitative real time PCR was used to detect the silence of miR-106b.The bladder cancer cells were divided into blank control group (group A),negative control group (group B),and miR-106b small-molecule inhibitor transfection group (group C).Cell proliferation assay and colony-forming assay were used to measure the effect of targeted inhibition of miR-106b on the growth of bladder cancer T24 cell,Annexin V/PI was used to measure the effect on the apoptosis of T24 cells,and cell migration assay and invasion assay were used to measure the effect on the invasion of in vitro T24 cells.Results There were significant differences in miR-106b expression,survival rate of cells,number of cell colonies formed,cell apoptosis rate,and number of cells across membrane between group C and groups A/B (F=37.24-87.89,q=9.5716.53,P＜0.05).Conclusion Targeted inhibition of miR-106b can inhibit the proliferation,invasion,and apoptosis of bladder cancer cells in vitro,and miR 106b is the target gene for the treatment of bladder cancer.关键词
膀胱肿瘤/miR-106b/细胞增殖/细胞凋亡Key words
urinary bladder neoplasms/miR-106b/proliferation/apoptosis分类
临床医学引用本文复制引用
赵凯,董大海,骆磊,王永华,杨晓坤..靶向抑制miR-106b对体外膀胱癌细胞增殖和凋亡影响[J].青岛大学医学院学报,2017,53(3):297-299,304,4.基金项目
山东省医药卫生科技发展计划项目(2014WS04-49) （2014WS04-49）
山东省高校科技计划项目(J16LL04) （J16LL04）
青岛市应用基础研究计划项目(15-9-1-105-jch) （15-9-1-105-jch）
