青岛大学医学院学报2017，Vol.53Issue(3)：308-311,4.DOI:10.13361/j.qdyxy.201703016
异丙酚后处理对大鼠脑外伤后AQP-4表达及神经元凋亡的影响
EFFECT OF PROPOFOL POST-TREATMENT ON AQUAPORIN-4 EXPRESSION AND NEURONAL APOPTOSIS AFTER BRAIN TRAUMA IN RATS
摘要
Abstract
Objective To investigate the effect of propofol on aquaporin-4 (AQP-4) expression and neuronal apoptosis after brain trauma in rats,as well as the mechanism of action of propofol in alleviating brain trauma.Methods A total of 150 healthy male Wistar rats were randomly divided into control group and propofol group,with 75 rats in each group.The Feeney method was used to establish a rat model of brain injury caused by free fall.The rats in the propofol group were given tail vein injection of 10 g/L propofol at a dose of 10 mg/kg at 1 h after injury,followed by micropump injection of propofol at a dose of 30 mg/(kg · h) for 2 h.The rats in the control group were given tail vein injection of an equal volume of normal saline at 1 h after injury,followed by micropump injection of an equal volume of normal saline for 2 h.Five rats each were randomly sacrificed at 6,12,24,48,and 72 h after injury,and the dry-wet weight method was used to measure the water content in the hemisphere.Ten rats were randomly selected at each time point and were treated with cardiac perfusion to collect brain tissue and prepare paraffin section;HE staining was used to observe the injury of brain tissue and the morphological changes of surrounding neurons,and TUNEL was used to measure the numbers of apoptotic neurons and AQP-4-positive cells in brain tissue.Results Compared with the control group,the propofol group had a significant reduction in cerebral water content at each time point (F=4.12-6.25,P＜0.05).As was shown by HE staining of brain tissue sections,the control group had increased intercellular space and perivascular space with disintegration and necrosis of glial cells and neurons in the area of brain trauma,swollen neuronal cell bodies and vascular endothelial cells in the adjacent area,and erythrocyte diapedesis and aggregation of inflammatory cells in some areas;compared with the control group,the propofol group had significant alleviation in these pathological changes and significant reductions in the numbers of necrotic and apoptotic cells.Compared with the control group,the propofol group had significant reductions in the numbers of AQP-4-positive cells and apoptotic neurons at each time point (F =4.02-9.56,P＜0.05);the propofol group had a significantly greater reduction in the number of AQP-4-positive cells at 12 and 24 h after injury (P＜0.05),as well as a significantly greater reduction in the number of apoptotic neurons at 24 and 48 h after injury (P＜0.05).Conclusion Propofol can downregulate the expression of AQP 4,reduce neuronal apoptosis after brain trauma,and thus alleviate brain injury.关键词
二异丙酚/脑损伤/水通道蛋白-4/细胞凋亡Key words
propofol/brain injurys/aquaporin 4/apoptosis分类
临床医学引用本文复制引用
谭海玲,宋建防,冀翔宇,周赞宫..异丙酚后处理对大鼠脑外伤后AQP-4表达及神经元凋亡的影响[J].青岛大学医学院学报,2017,53(3):308-311,4.基金项目
青岛市科技局科研基金资助项目(10-3-4-7-8-JCH) （10-3-4-7-8-JCH）
