热带作物学报2017，Vol.38Issue(5)：783-791,9.DOI:10.3969/j.issn.1000-2561.2017.05.001
剑麻种质资源表型多样性分析与倍性鉴定
Morphological Diversity and Ploidy Identification in Agave Germplasms
摘要
Abstract
Ten morphological traits including leaf color,have/none marginal spine,apical spine type,leaf length,leaf width,length/width of leaf,thickness of leaf base,length of apical spine,and spine basal width were examined,and ploidy level was determined by an analysis of flow cytometry.SPSS statistical analysis for leaf length,leaf width,length/width of leaf,thickness of leaf base,length of apical spine,spine basal width revealed that The coefficient of variation within Agave germplasms was 0.9％-13.33％,1.69％-9.13％,1.77％-7.61％,3.99％-11.62％,7.82％-14.31％,9.08％-14.84％,among Agave germplasms was 21.60％,13.95％,15.9％,21.48％,36.59％,30.77％ respectively.ANOVA showed that ten morphological traits were statistically different among 25 Agave germplasms (α=0.05),but no different within germplasm (α＞0.05).Cluster analysis based on complete distance with SAS of ten morphological traits showed that 25 germplasms were divided into three groups,A.americana L,Guangxi76416,Nanya 1,ML387,A.tequilana F.A.C weber,A.sisalana perrine,and Yuexi75 were scattered into the first group,Guifu4,H.11648,Dong74,Dong27,Dong26,Dongl6 were clustered into the second group,the other 12 germplasms were scattered into the third group.The principal component analysis results indicated that the sum of eigenvalue of the first 4 principal components was 5.84,and the contribution rate accounted for 97.29％.Among the 25 Agave germplasms,14 germplasm such as H.11648 and Dong2 are diploids,Dong16 and Guangxi76416 are triploids,5 germplasms such as Yuexi75 and Yuexi 114 are tetraploids,Ken3 and A.sisalana Perrine are pentaploids,Yuexi74 is a hexaploid,A.americana L.is a mixoploid of pentaploid and hexaploid.The results would provide basic reference on Agave germplasm identification,and classification.关键词
剑麻/表型多样性/倍性鉴定/流式细胞术Key words
Sisal/morphological diversity/ploidy identification/flow cytometry分类
农业科技引用本文复制引用
张燕梅,石胜友,李俊峰,鹿志伟,陆军迎,周文钊..剑麻种质资源表型多样性分析与倍性鉴定[J].热带作物学报,2017,38(5):783-791,9.基金项目
国家自然科学基金(No.31401427) （No.31401427）
海南省自然科学基金(No.314112) （No.314112）
国家麻类产业技术体系项目(No.CARS-19) （No.CARS-19）
湛江市热带作物遗传改良重点实验室建设项目(No.2015A06005). （No.2015A06005）
