热带作物学报2017，Vol.38Issue(5)：858-863,6.DOI:10.3969/j.issn.1000-2561.2017.05.013
辣木叶γ-氨基丁酸提取工艺及其抗氧化研究
Optimization of Extraction of γ-aminobutyric Acid from Moringa oleifera Leaves and Their Antioxidant Activities
摘要
Abstract
To optimize the extraction conditions of γ-aminobutyric acid from Moringa oleifera leaves and evaluate the antioxidant activity,univariate experiments and orthogonal experiments design were employed to study the effect of different extraction temperature,the ratio of liquid to material and extraction time on the extraction efficiency of γ-aminobutyric acid from Moringa oleifera leaves.Meanwhile,the results showed that the optimized parameters of extraction were extraction temperature of 60 ℃,the ratio of liquid and material of 1∶15,and extraction time of 30 min.Under these conditions,the γ-aminobutyric acid content was (3.40±0.09)mg/g.The crude extracts obtained from M.oleifera leaves had strong scavenging capacity on DPPH,.OH and ABTS free radical in a dose-dependent manner.The EC50 values of crude extracts were 0.149 0 mg/mL,0.092 5 mg/mL and 0.039 8 mg/mL,respectively.关键词
辣木叶/提取/γ-氨基丁酸/抗氧化活性Key words
Moringa oleifera leaves/extraction/γ-aminobutyric acid/antioxidant activity分类
农业科技引用本文复制引用
刘能,周伟,林丽静,李积华,郭长青,雷少玲,陈军..辣木叶γ-氨基丁酸提取工艺及其抗氧化研究[J].热带作物学报,2017,38(5):858-863,6.基金项目
广西科学研究与技术开发计划(No.桂科攻15248003-18) （No.桂科攻15248003-18）
中国热带农业科学院院本级基本科研业务费专项资金(No.1630062015015) （No.1630062015015）
湛江市科技计划项目(No.2015A03024). （No.2015A03024）
