热带作物学报2017，Vol.38Issue(5)：894-902,9.DOI:10.3969/j.issn.1000-2561.2017.05.018
茶树几丁质酶基因的克隆及其在干旱胁迫下的表达分析
Cloning and its Expression Analysis of Chitinase Under Drought Stress in Camellia sinensis
摘要
Abstract
The chitinase gene CsChi (GenBank accession number:KR078345) was cloned by the RT-PCR method combined with RACE technique from the leaves of tea plant.The full length cDNA of CsChi was 1 192 bp containing an open reading frame (ORF) of 972 bp encoding 323 amino acids.Bioinformatics predicted that the molecular mass of CsChi protein was 34.33 ku.The theoretical pI was 8.44.The atomic composition was C1519H2 285N413O464S18 and the total number of atoms was 4 699.The protein structure analysis showed that the protein had 6 transmembrane regions,which belonging to transmembrane protein.The protein existed the outside cell and had no coiled-coil structure.The deduced protein belonged to glycoside hydrolase family 19 and had a conserved ChtBD1 domains which had a high identity with the lysozyme,and it may be a bifunctional enzyme of chitinase and lysozyme.Analysis by qPCR showed that the transcript of CsChi under different drought stresses was up-regulated higher than the control group.We speculate that CsChi gene plays an important role in adversity stresses such as drought stress.关键词
茶树/几丁质酶/基因克隆/表达分析Key words
Camellia sinensis/chitinase/gene cloning/expression analysis分类
轻工纺织引用本文复制引用
朱晨,郭玉琼,张舒婷,常笑君,赵姗姗,王仲,许长同,张梓浩,林玉玲,赖钟雄..茶树几丁质酶基因的克隆及其在干旱胁迫下的表达分析[J].热带作物学报,2017,38(5):894-902,9.基金项目
福建省自然科学基金(No.2013J01085) （No.2013J01085）
福建省重大科技专项(No.2015NZ0002-1) （No.2015NZ0002-1）
福建省教育厅计划项目(No.JA15143). （No.JA15143）
