热带作物学报2017，Vol.38Issue(5)：919-925,7.DOI:10.3969/j.issn.1000-2561.2017.05.022
42℃极端高温胁迫对木薯单爪螨(Mononychellus mcgregori)保护酶及热激蛋白基因表达的影响
Influence of Extreme High Temperature Exposure on Protective Enzymes and Hsp70 Gene Expression of Mononychellus mcgregori (Acari: Tetranychidae)
摘要
Abstract
Cassava green mite Mononychellus mcgregori is a newly invasive pest mite of cassava in China.It was reported the first time in Danzhou,Hainan,China in 2008 and has since become more widespread.The origin of M.mcgregori is South America and as a tropical native mite,the adaptation to high temperature is the significant foundation for its occurrence and damage.The results showed that the relative expression of PPO,POD,AsA-POD,CA T and Cu/Zn SOD genes in M.mcgregori via RTFQ PCR increased in adults after they were exposed to extremely high temperature (42 ℃) for 1 h,which increased 5.19,4.38,152.23,321.42 and 3.41 times over that of the control,respectively,and were in accord with their enzyme activities (1.81,12.52,9.00,1.75 and 1.14 times over that of the control).And the relative expression of heat shock protein 70 (Hsp70) gene increased 56.85 times over that of the control.These results suggested that protective enzymes POD,AsA-POD,CAT and SOD were associate with the thermostability of M,mcgregori and they might have synergistic effects on protect the mite against toxic oxygen intermediates and perhaps were associate with the resistance of high temperature in M.mcgregori.The increasing of PPO gene expression was perhaps associated with the immunity improvement of the mite to high temperature stress.And the elevated expression of Hsp70 gene was perhaps related to the adaptation of M.mcgregori to high temperature.These results provide a theoretical basis and precondition for further research on the molecular mechanisms of the thermostability and ecological adaptability of M.mcgregori.关键词
木薯单爪螨/高温/保护酶/热激蛋白/基因表达/酶活性Key words
Mononychellus mcgregori/extreme high temperature/protective enzymes/heat shock protein/gene expression/enzyme activities分类
农业科技引用本文复制引用
卢芙萍,陈青,伍春玲,卢辉,梁晓..42℃极端高温胁迫对木薯单爪螨(Mononychellus mcgregori)保护酶及热激蛋白基因表达的影响[J].热带作物学报,2017,38(5):919-925,7.基金项目
国家木薯产业技术体系害虫防控岗位科学家专项(No.CARS-12-hncq) （No.CARS-12-hncq）
中国热带农业科学院环境与植物保护研究所基本科研业务费专项(No.2015hzs1J011) （No.2015hzs1J011）
海南省科协青年科技英才学术创新计划项目(No.201508). （No.201508）
