热带作物学报2017，Vol.38Issue(5)：950-956,7.DOI:10.3969/j.issn.1000-2561.2017.05.027
菠萝蜜类胡萝卜素检测方法研究及呈色物质分析
Method of Carotenoids Analysis and Pigments Identification in Jackfruit
摘要
Abstract
In order to identify the carotenoid composition and pigments in jackfruit,the High Performance Liquid Chromatography (HPLC) was applied to analyze the composition and concentration of carotenoids in different fruit samples.By homogenizing in extracting solution (n-Hexane∶acetone∶ethanol=2∶1∶1,Ⅴ/Ⅴ/Ⅴ) with 0.01％ butylated hydroxytoluene (BHT),accompanied with sonication and saponification,the total carotenoid in jackfruit was extracted.The analysis was carried out in a Waters liquid chromatography system equipped with a model 2996 photodiode array detector (PAD).A C30 carotenoid column (250×4.6 ram;YMC,Japan) was used to elute the carotenoids.Sixteen carotenoids were successfully separated,identified and quantified by the HPLC-PDA system.In red-flesh fruit,the main carotenoids were luteoxanthin,3-citraurin,violaxanthin and all-trans-neoxanthin.β-citraurin was the only red substance,which determined the color of red-flesh fruit.In yellow-flesh fruit,the main carotenoid was violaxanthin.In summary,the cleavage or epoxidase of the precursors of violaxanthin,and isomerization of violaxanthin might determined the different color of jackfruit.Our study provide an efficient method for carotenoids analysis in jackfruit.Additionally,the relationship between pigments suggest a new insight of carotenoid metabolism,which would provide a regulatory mechanism by which to facilitate improvements to fruit quality in jackfruit.关键词
菠萝蜜/果肉色泽/类胡萝卜素/呈色组分鉴定Key words
Jackfruit/flesh color/carotenoids/pigment identification分类
农业科技引用本文复制引用
胡丽松,吴刚,郝朝运,范睿,伍宝朵,谭乐和..菠萝蜜类胡萝卜素检测方法研究及呈色物质分析[J].热带作物学报,2017,38(5):950-956,7.基金项目
2017年海南省自然科学基金面上项目-红色果肉菠萝蜜呈色物质鉴定及关键基因克隆(No.317273) （No.317273）
中国热带农业科学院基本科研业务费专项资金-热带木本粮食作物资源与产业关键技术研究(No.1630142016003). （No.1630142016003）
