热带作物学报2017，Vol.38Issue(5)：962-968,7.DOI:10.3969/j.issn.1000-2561.2017.05.029
海南粗榧内生真菌F127发酵液的次级代谢产物研究
Secondary Metabolites of Endophyte F127 from Cephalotaxus hainanensis Li.
摘要
Abstract
To study the chemical constituents of the endophyte F127 of Cephalotaxus hainanensis Li.,the constituents were isolated and purified by multi-chromatography,and their structure were elucidated by spectroscopic analyses including LC-MS,ESI-MS,1H NMR and 13C NMR.Meanwhile,the cyctotoxic activities were evaluated by MTT assay.Five known metabolites including oblongolide T(1),sorbicillin(2),dibutylphthalate(3),phomopsolide B(4) and one 3,4-dihydroisocomnarin(5) were isolated from the broth of the endophyte F127.And compound 1,4,5 exhibited different cyototoxic activities against K562,NB4,HL-60,HepG-2 and LoVo.Compound 4 showed cytotoxic activities against 5 tumor cell lines in vitro,which significantly inhibiting K562,NB4,HL-60 cells with IC5o of 3.35,0.014 and 0.16 μg/mL,and moderately inhibiting HepG-2 and LoVo.Compound 1 exhibited good cytotoxic activites aginst K562,NB4,HL-60 cells as well,with IC5o of 51.82,54.25 and 29.31 μg/mL.Moderate cytotoxic activites aginst NB4 and HepG-2 were alsso observed for compound 5.The rest of the founds showed no substantial effect against the tumor cells.The five compounds were all the first time isolated from the endophytes from C.hainanensis Li..And the significant cytotoxic activtities against to K562,NB4,HL-60 of compound 1 and 4 was reported for the first time,which laid the foundation of studying the bioactive natural products from endophytes from C.hainanensis Li..关键词
海南粗榧/内生真菌/天然产物/抗肿瘤活性/phomopsolide/oblongolideKey words
Cephalotaxus hainanensis/endophyte/natural products/antitumor activity/phomopsolide/oblongolide分类
医药卫生引用本文复制引用
李培,阳辉蓉,胡晓苹,李从发,徐传标,马敏珺,刘四新..海南粗榧内生真菌F127发酵液的次级代谢产物研究[J].热带作物学报,2017,38(5):962-968,7.基金项目
海南省自然科学基金项目(No.313034) （No.313034）
中国博士后科学基金(No.90279). （No.90279）
