山东农业科学2017，Vol.49Issue(8)：33-37,5.DOI:10.14083/j.issn.1001-4942.2017.08.008
头孢霉素与卡那霉素对花生组培苗生根的影响
Effects of Cephalosporin and Kanamycin on Rooting of Peanut Tissue Culture Seedlings
摘要
Abstract
Resistance screening is an indispensable step in Agrobacterium mediated genetic transformation of peanut,but the use of antibiotics have significant influence on peanut seedling growth and rooting.In order to make clear the sensitive concentration of antibiotics on rooting of peanut seedlings,the peanut cultivation variety Fenghua 1 tissue culture seedlings were used as experimental materials,and the culture media with different concentrations of kanamycin or cefotaxime were used to detect the peanut seedling rooting rate and growth status.The results showed that 0~250 mg/L cefotaxime had little effect on peanut growth,while kanamycin had significant effect on peanut seedling rooting.0~10 mg/L kanamycin has no obvious effect on peanut seedling rooting and growth,but under the condition of 10 mg/L kanamycin,the seedlings had no branch roots,what''s more,15 mg/L kanamycin significantly inhibited the elongation of roots.When the rootless plantlets were transfered into MS medium after cutting off the bottom of the seedlings,the roots grew out again,whereas the rootless plantlets did not grow roots if not cutting off the bottom.After screening with 75 mg/L kanamycin,the rooting rate of the kanamycin-resistant tissue culture plantlets could reach 31%.This study could provide certain theoretical supports for roots induction during the late stage of peanut genetic transformation.关键词
头孢霉素/卡那霉素/花生/组培苗/生根率/遗传转化Key words
Cefotaxime/Kanamycin/Peanut/Tissue culture seedling/Rooting rate/Genetic transformation分类
农业科技引用本文复制引用
阮建,郭峰,李新国,史灵敏,田海莹,张会,万书波,彭振英..头孢霉素与卡那霉素对花生组培苗生根的影响[J].山东农业科学,2017,49(8):33-37,5.基金项目
国家自然科学基金项目(31571605) （31571605）
国家科技支撑计划项目(2014BAD11B04) （2014BAD11B04）
国家国际科技合作专项(2015DFA31190) （2015DFA31190）
现代农业产业技术体系建设专项(CARS-14) （CARS-14）
