水生生物学报2017，Vol.41Issue(5)：956-962,7.DOI:10.7541/2017.119
在患CyHV-2病的异育银鲫肠道黏膜中胆固醇、胆汁酸代谢通路基因的差异表达
GENE DIFFERENCE EXPRESSION OF CHOLESTEROL AND BILE ACID METABOLISM PATHWAY IN INTESTINAL MUCOSA WITH THE CYHV-2 DISEASE CARASSIUS AURATUS GIBELIO
摘要
Abstract
Gibel carp (Carassius auratus gibelio) infected with Cyprinid herpesvirus 2 (CyHV-2), damaged many or-gans including intestinal mucosa. Total RNA of intestinal mucosa from CyHV-2 diseased and normal gibel carp were extracted for RNA-Seq. Transcriptome assembly from RNA-Seq data without a reference genome was conducted by Trinity (version: v2012-10-05, min_kmer_cov 2) software. Single gene is annotated by using different databases. Soft-ware and the database of the functional gene annotation are SwissProt (NCBI blast 2.2.27+, Blast2GO v2.5), KOG (NCBI blast 2.2.27+), PFAM (HMMER 3.0 package), KEGG (KOBAS, NCBI blast 2.2.27+). Gene expression was analyzed by edgeR package. 3335 up-regulated genes and 4435 down-regulated genes were observed, indicating a sig-nificant injury in the intestinal mucosa by CyHV-2 infection. The metabolic disorder for enterohepatic circulation of bile acids was supported by the reduced gene expression that regulate cholesterol and bile acid synthesis metabolism and other physiological processes, secretion, absorption and transportation. As a consequence, CyHV-2 infection in Crucian carp decreased the serum bile acid content by 99％ and cholesterol content by 10％.关键词
氧化鱼油/黏膜/胆固醇/胆汁酸/异育银鲫Key words
Oxidized fish oil/Mucosa/Cholesterol/Bile acid/Gibel carp分类
农业科技引用本文复制引用
叶元土,吴萍,蔡春芳,林秀秀,吴代武,何杰,张宝彤,萧培珍..在患CyHV-2病的异育银鲫肠道黏膜中胆固醇、胆汁酸代谢通路基因的差异表达[J].水生生物学报,2017,41(5):956-962,7.基金项目
江苏省水产三新工程项目(D2015-12)资助 Supported by the Jiangsu Province, Three New Aquatic Projects (D2015-12) Funding （D2015-12）
