水生生物学报2017，Vol.41Issue(5)：963-971,9.DOI:10.7541/2017.120
黄颡鱼20β-羟基类固醇脱氢酶Ⅰ和Ⅱ基因特征分析和表达模式研究
CHARACTERATION OF THE DUPLICATED 20β-HSD GENES IN PELTEOBAGRUS FULVIDRACO AND THEIR EXPRESSION PATTERNS AND REGULATIONS
摘要
Abstract
Maturation inducing hormone (MIH) plays pivotal roles in gamete maturation, ovulation and spermiation of fish. In most teleost species, 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) is the major MIH. The steroidogenic enzyme, named 20β-hydroxysteroid dehydrogenase (20β-HSD), is responsible for DHP production in several teleost species. To date, only one copy of 20β-hsd has been characterized in vertebrates, including fish. In this study, two copies of 20β-hsd genes were identified in genomes of several fish species. Phylogenetic and syntenic analyses revealed that the du-plication of 20β-hsd gene may not result from the teleost specific genome duplication (TSGD). Multiple-alignment re-sults indicated that these two enzymes might possess similar spatial structures and co-enzyme/substrate binding motifs, whereas, their catalytic activities might differ. The duplicated copy of 20β-hsd was further cloned from yellow catfish (Pelteobagrus fulvidraco). Tissue distribution results showed that, 20β-hsd Ⅰ mRNA was mainly expressed in testis, ovary, head kidney and kidney, while 20β-hsd Ⅱ mRNA was mainly located in testis, ovary, heart, head kidney, kid-ney, intestine, pituitary and liver. Seasonal expression results revealed that, lower 20β-hsd Ⅰ expression and higher 20β-hsd Ⅱ expression were observed in male fish during reproductive time window (in May). Three hours after injec-tion of hCG (1000 IU/kg body weight), the expression of 20β-hsd Ⅰ was up-regulated and then suppressed signifi-cantly, while the expression of 20β-hsd Ⅱ was up-regulated in a time dependent manner in male fish. These results demonstrated the differential regulation pattern of 20β-hsd Ⅰ and 20β-hsd Ⅱ in response to hCG.关键词
黄颡鱼/20β-HSD/复制基因/cDNA克隆/表达模式/hCG处理Key words
Pelteobagrus fulvidraco/20β-HSD/Duplicated gene/cDNA cloning/Expression pattern/hCG treatment分类
生物科学引用本文复制引用
尹盼,李英文,王雅琴,刘智皓..黄颡鱼20β-羟基类固醇脱氢酶Ⅰ和Ⅱ基因特征分析和表达模式研究[J].水生生物学报,2017,41(5):963-971,9.基金项目
重庆市科委项目 (cstc2016jcyjA0133) （cstc2016jcyjA0133）
重庆市教委项目 (KJ130622和KJ1600308) （KJ130622和KJ1600308）
重庆师范大学校级项目(12XLB005和13XLZ08)资助 Supported by the Natural Science Foundation Project of CQ CSTC (cstc2016jcyjA0133) （12XLB005和13XLZ08）
Ministry of Education Project of CQ (KJ130622, KJ1600308) （KJ130622, KJ1600308）
the Key Project of the CQNU (12 XLB005, 13XLZ08) （12 XLB005, 13XLZ08）
