池蝶蚌β-连环蛋白基因cDNA的克隆及表达特征分析OA北大核心CSCDCSTPCD
THE STRUCTURAL FEATURE AND EXPRESSION ANALISIS OF HSβ-CATENIN cDNA AND PROTEIN FROM HYRIOPSIS SCHLEGELII
为了解淡水贝类性别调控与分化机制,课题组建立了池蝶蚌(Hyriopsis schlegelii)性腺转录组,在转录组库中,存在β-连环蛋白(β-catenin)基因序列.实验对池蝶蚌β-catenin基因进行验证,采用RACE技术克隆其cDNA全长,命名为Hsβ-catenin.该序列全长4386 bp,5′-非编码区为162 bp,3′-非编码区为1758 bp,开放阅读框为2466 bp,编码821个氨基酸;该蛋白结构域主要由12个ARM重复…查看全部>>
In order to explore the mechanism of sexual development of freshwater shellfish, we established a gonadal transcriptome of Hyriopsis schlegelii. Based on the transcriptome library, this study investigated the β-catenin. The full-length cDNA was cloned by RACE PCR, which named Hsβ-catenin. The full length of Hsβ-catenin cDNA was 4386 bp that contained an open reading frame (ORF) of 2466 bp coding a protein of 821 amino acids. The protein was mainly composed o…查看全部>>
闫含笑;史建伍;盛军庆;王军花;洪一江
南昌大学生命科学学院,南昌 330000南昌大学生命科学学院,南昌 330000南昌大学生命科学学院,南昌 330000南昌大学生命科学学院,南昌 330000南昌大学生命科学学院,南昌 330000
生物科学
池蝶蚌Hsβ-catenin组织表达克隆序列分析qRT-PCR
Hyriopsis schlegeliiHsβ-cateninTissue expressionCloneSequence analysisqRT-PCR
《水生生物学报》 2017 (5)
池蝶蚌性别分化相关环境因子及Fem-1和Tra-2基因家族的表达调控研究
972-976,5
国家自然科学基金(31660337) 江西省教育厅项目(GJJ150166)资助 Supported by the National Natural Science Foundation of China (31660337) the Fund Project of Jiangxi Provincial Education Department (GJJ150166)
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