水生生物学报2017，Vol.41Issue(5)：972-976,5.DOI:10.7541/2017.121
池蝶蚌β-连环蛋白基因cDNA的克隆及表达特征分析
THE STRUCTURAL FEATURE AND EXPRESSION ANALISIS OF HSβ-CATENIN cDNA AND PROTEIN FROM HYRIOPSIS SCHLEGELII
摘要
Abstract
In order to explore the mechanism of sexual development of freshwater shellfish, we established a gonadal transcriptome of Hyriopsis schlegelii. Based on the transcriptome library, this study investigated the β-catenin. The full-length cDNA was cloned by RACE PCR, which named Hsβ-catenin. The full length of Hsβ-catenin cDNA was 4386 bp that contained an open reading frame (ORF) of 2466 bp coding a protein of 821 amino acids. The protein was mainly composed of 12 ARM repeat sequences. In the secondary structure, the α-helix accounted for 47.75％ and the β-folding accounted for 1.22％ were predicted. The tertiary structure had many α-helixs, namely ARM domain. Hsβ-catenin gene was clustered with homologous genes from soft animal in the phylogenetic tree. Hsβ-catenin expressed highly in intes-tine and modestlyin foot and testis. The expression level of Hsβ-catenin was high at the age of 12-month-old (gender differentiation) and 36-month-old (gonad mature), indicated that it might be involved in sexual development.关键词
池蝶蚌/Hsβ-catenin/组织表达/克隆/序列分析/qRT-PCRKey words
Hyriopsis schlegelii/Hsβ-catenin/Tissue expression/Clone/Sequence analysis/qRT-PCR分类
生物科学引用本文复制引用
闫含笑,史建伍,盛军庆,王军花,洪一江..池蝶蚌β-连环蛋白基因cDNA的克隆及表达特征分析[J].水生生物学报,2017,41(5):972-976,5.基金项目
国家自然科学基金(31660337) （31660337）
江西省教育厅项目(GJJ150166)资助 Supported by the National Natural Science Foundation of China (31660337) （GJJ150166）
the Fund Project of Jiangxi Provincial Education Department (GJJ150166) （GJJ150166）
