水生生物学报2017，Vol.41Issue(5)：992-999,8.DOI:10.7541/2017.124
黄颡鱼MHC classⅡ抗原基因全长的克隆及饲料维生素D3对其组织表达的影响
FULL-LENGTH cDNA CLONING AND THE EFFECT OF DIETARY VITAMIN D3 ON TISSUE EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX-Ⅱ IN YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)
摘要
Abstract
To study the relationship between vitamin D3 (VD3) and the major histocompatibility complex (MHC) class Ⅱ gene, the MHC class Ⅱ antigen gene of yellow catfish was cloned by rapid-amplification of cDNA ends. The full-length cDNA of MHC class Ⅱ is 1074 bp long and comprises a 78 bp 5′ untranslated region (UTR_, 259 bp 3′UTR, and a 708 bp open reading frame that encodes 236 amino acids. The deduced amino acid sequence showed the highest iden-tity (69.5％) and the lowest identity (50.4％) with Leiocassis longirostris and Cyprinus carpio, respectively. Expression of the MHC class Ⅱ antigen gene was relatively high in the small intestine, liver, and gill, but low in muscles and tail. It was nearly undetectable in the other tissues. Vitamin D3 (0, 5000 IU/kg) was added to low phosphorus diets in the growth trial. The addition of dietary VD3 significantly stimulated MHC class Ⅱ gene expression in the small intestine and kidney. This study describes the molecular structure, tissue expression of the MHC class II antigen gene, and pos-sible effects of dietary VD3 on tissue expression of the MHC class Ⅱ gene in yellow catfish, providing useful informa-tion for further studies of the function of this gene in yellow catfish.关键词
黄颡鱼/MHCclassⅡ/饲料维生素D3/基因表达Key words
Yellow catfish/MHC class Ⅱ/Dietary VD3/Gene expression分类
农业科技引用本文复制引用
冯美惠,陈沛,雷文,李大鹏,王春芳..黄颡鱼MHC classⅡ抗原基因全长的克隆及饲料维生素D3对其组织表达的影响[J].水生生物学报,2017,41(5):992-999,8.基金项目
国家自然科学基金(31172421和31672667) （31172421和31672667）
现代农业产业技术体系建设专项基金(CARS-46)资助 Supported by the National Natural Science Foundation of China (31172421, 31672667) （CARS-46）
the Earmarked Fund for China Agriculture Research System (CARS-46) （CARS-46）
