水生生物学报2017，Vol.41Issue(5)：1020-1026,7.DOI:10.7541/2017.127
氧化鱼油对黄颡鱼黑色素合成酶及内分泌激素的影响
EFFECTS OF DIETARY INCLUSION OF OXIDIZED FISH OIL ON MELANIN, MELANIN SYNTHETIC ENZYMES AND HORMONES OF PELTEOBAGRUS FULVIDRACO
摘要
Abstract
We investigated the effects of fish feed containing oxidized fish oil on epidermal melanin expression, tyro-sinase activity, and hormone levels related to melanin synthesis in the serum and brain of Pelteobagrus fulvidraco. Fish oil was oxidized under natural conditions and substituted for normal fish oil in fish feed at concentrations of 100％(group Y100), 50％ (group Y50), and 0 (group C). Level 5 melanin cells, in which melanin accounts for >80％ of the cell area, increased in a dose-dependent manner with the amount of oxidized fish oil in the feed. There was a tendency for tyrosinase activity to increase with the amount of oxidized fish oil in the fish feed, but the increase was not statisti-cally significant. Brain tissue adreno-cortico-tropic hormone (ACTH) increased with the amount of oxidized oil in feed, while it only increased significantly in the serum of the Y50 group. Serum corticotropin releasing hormone decreased significantly in both the Y50 and 100 treatment groups but showed no significant changes in brain tissues. Similarly, serum α-melanocyte stimulating hormone (α-MSH) in group Y100 was significantly elevated, but no significant diffe-rences were detected in brain tissues. In contrast, serum and brain tissue corticosterone levels showed no significant dif-ferences among the three groups. These results suggest that oxidized fish oil in fish feed was positively related to the proportion of level 5 melanin cells and epidermal tyrosinase activity. Such changes were related to the increase in ACTH and α-MSH in P. fulvidraco.关键词
黄颡鱼/氧化鱼油/黑色素/酪氨酸酶/内分泌激素Key words
Pelteobagrus fulvidraco/Oxidized fish oil/Melanoma/Tyrosinase/Hormone分类
生物科学引用本文复制引用
程炜轩,许国焕,张丽,韩木兰,陈金涛,魏逸峰..氧化鱼油对黄颡鱼黑色素合成酶及内分泌激素的影响[J].水生生物学报,2017,41(5):1020-1026,7.基金项目
广东省海洋渔业科技与产业发展专项科技攻关与研发项目 (A201401B05) （A201401B05）
2015年广州市科技计划项目 (一般项目) (201510010239) （一般项目）
广东省科学院引进高层次领军人才专项资金(2016GDASRC-0202)资助 Supported by the Science and Technology Special Development Program for Guangdong Province Marine Fishy Science, Technology and Industrial Development (A201401B05) （2016GDASRC-0202）
the Science and Technology Planning Project of Guangdong Province in 2015 (201510010239) （201510010239）
the High-level Leading Talent Introduction Program of GDAS (2016GDASRC-0202) （2016GDASRC-0202）
