水生生物学报2017，Vol.41Issue(5)：1042-1047,6.DOI:10.7541/2017.130
凡纳滨对虾血蓝蛋白酶解多肽的凝集与抑菌活性
AGGLUTINATIVE AND ANTIBACTERIAL ACTIVITY OF THE PEPTIDES HYDROLYZED FROM LITOPENAEUS VANNAMEI HEMOCYANIN WITH TRYPSIN
摘要
Abstract
Previous studies have demonstrated antibacterial and antifungal activities of hemolymph-derived hemocyan-in upon pathogen infection with unknown mechanisms. To investigate how peptides derive from hemocyanin, multiple methods including size-exclusion chromatography, Tricine-SDS-PAGE, immunoblotting, bacterial agglutinative and antibacterial assays were applied. The results showed that shrimp Litopenaeus vannamei hemocyanin could produce seven peptides via trypsin digestion, ranging from 6 to 70 kD, which could be identified by rabbit anti-shrimp hemoc-yanin antibody specifically. These peptides aggregated with Vibrio parahaemolyticus in vitro, whose agglutinative acti-vity was 4—16 folds higher than that of full length hemocyanin. The peptide 3 possessed obvious antibacterial activit-ies against V. parahaemolyticus with a antibacterial rate (93.76±1.60)％ at the concentration of 75 μg/mL, which was significantly higher than that of the control group (P<0.01). In addition, N-terminal Edman Sequencing analysis showed that the peptide 3 was located in the α-helix region of N-terminus of shrimp L. vannamei hemocyanin small subunit. These discoveries will help to understand how the hemocyanin derived peptides are formed and to establish effective strategies for shrimp disease control.关键词
凡纳滨对虾/血蓝蛋白/胰蛋白酶/酶解多肽/凝集/抑菌Key words
Litopenaeus vannamei/Hemocyanin/Trypsin/Hydrolyzed peptides/Agglutinative/Antimicrobial分类
农业科技引用本文复制引用
李长平,黄河,王帆,钟名其,陈洁辉,章跃陵..凡纳滨对虾血蓝蛋白酶解多肽的凝集与抑菌活性[J].水生生物学报,2017,41(5):1042-1047,6.基金项目
国家自然科学基金 (31372558) （31372558）
广东省自然科学基金(S2013010015190) （S2013010015190）
广东省高等学校重大科研项目培育计划类项目(2014GKXM043)资助 Supported by National Natural Science Foundation of China (31372558) （2014GKXM043）
Natural Science Foundation of Guangdong Province (S2013010015190) （S2013010015190）
National Key Growth Project of Guangdong Province College (2014GKXM043) （2014GKXM043）
