生态学报2017，Vol.37Issue(15)：5146-5156,11.DOI:10.5846/stxb201605050863
两种代表性蚯蚓对设施菜地土壤微生物群落结构及理化性质的影响
Impacts of two typical earthworms on soil microbial community structure and physicochemical properties in a greenhouse vegetable field
摘要
Abstract
Earthworms are considered one of the most important soil ecosystem engineers.Earthworms play critical roles in soil functioning through their burrowing,feeding,and casting activities.There are distinct ecological strategies (i.e.,food selection,ingestion,assimilation,and habitat preference) for different earthworm ecotypes.Consequently,different earthworm ecotypes would have different effects on soil properties.Understanding the impacts of different earthworms on soil microbial communities would increase our mechanistic understanding of the role of earthworms in ecosystem services.Several studies have shown that earthworms facilitate the translocation of fresh residues into soil and create suitable habitat (casts,middens and burrows) for other soil organisms.Moreover,the interaction of earthworm feeding strategies and organic amendment could profoundly modulate soil properties and microbial community structure.However,there is a knowledge gap in this respect.In particular,few studies have been conducted under field conditions.To understand the long-term impact of earthworms and manure on soil microbial community structure,a tomato-spinach rotation experiment was initiated in 2009.Based on the soil collected during the fourth year following the set-up of the field experiment,the effects of two typical earthworms (i.e.,endogeic Metaphire guillelmi and epigeic Eisenia foetida) and two manure application treatments (i.e.,surface mulch and incorporation into the soil) on soil microbial community were investigated.The native endogeic species,Metaphire guillelmi,lives in the upper soil layers and primarily consumes soil mineral particles rich in organic matter,whereas E.foetida,a well-known epigeic species,resides mainly in the upper organic layers and feeds on litter materials.Our results demonstrated that soil microbial community structure was significantly affected by earthworm species and the method of manure application.With manure application at the soil surface,earthworms could significantly decrease mycorrhizal fungi,fungi biomass and protozoa biomass independent of earthworm type (P ＜ 0.05).When manure was incorporated into the soil,M.guillelmi significantly increased mycorrhizal fungi,fungi biomass and actinomyces biomass,although the effect of E.foetida was not significant.Regardless of type,earthworms significantly increased soil porosity,soil aggregate stability represented by mean weight diameter and pH,soil mineral nitrogen,available phosphorus,as well as microbial biomass carbon and nitrogen content.In addition,the extent of increase depended on earthworm ecotype and the method of manure application.Redundancy analysis showed that earthworm-mediated microbial community structure was positively correlated with soil aggregate stability as expressed by mean weight diameter,pH,and available phosphorus eontent,whereas it was negatively correlated with soil bulk density.关键词
微生物群落/赤子爱胜蚓/威廉腔环蚓/有机物施用/土壤性质/土壤动物Key words
microbial community/Eisenia foetida/Metaphire guillelmi/organic amendment/soil properties/soil fauna引用本文复制引用
王笑,王帅,滕明姣,林小芬,吴迪,孙静,焦加国,刘满强,胡锋..两种代表性蚯蚓对设施菜地土壤微生物群落结构及理化性质的影响[J].生态学报,2017,37(15):5146-5156,11.基金项目
国家自然科学基金项目(41171206) （41171206）
公益性行业科研专项(201503121) （201503121）
江苏省高校优势学科建设工程项目(PAPD) （PAPD）
