天然产物研究与开发2017，Vol.29Issue(7)：1085-1090,1147,7.DOI:10.16333/j.1001-6880.2017.7.001
蝙蝠蛾拟青霉与放线菌共培养对次生代谢产物的影响
Co-culture of Paecilomyces hepiali with Actinomycete and its Effect on the Secondary Metabolites
摘要
Abstract
To explore the production of induced secondary metabolites by a co-culture of Paecilomyces hepiali and actinomycete.A strain of actinomycete TY134 with strong antibacterial activity was isolated from the soil microbial flora of Ophiocordyceps sinensis.This actinomycete was identified as Streptomycesfulvissimus on the basis of 16S rDNA.The monoculture of P.hepiali,actinomycete TY134 respectively,and the co-culture were done on rice solid medium for 30 days,then the fermentation products were extracted by the aid of ultrasonic with ethyl acetate respectively.Comparison of the chemical composition and anti-microbial activity of the ethyl acetate extract of monoculture and co-culture were performed by HPLC and double drilling method respectively.The results showed that the co-culture of ethyl acetate extract produced a new absorption peak at the retention time 14.143 min,and there was no evidence of the absorption peak in monoculture.In addition,the anti-bacterial activity of the ethyl acetate extract of the co-culture was stronger than that of the monoculture,produce chemical components with new anti-microbial activities.All the results indicated that co-culture can be used as a promising approach to discover new bioactive secondary metabolites.关键词
蝙蝠蛾拟青霉/放线菌/共培养/单培养/次生代谢产物Key words
Paecilomyces hepiali/actinomycete/co-culture/monoculture/secondary metabolites分类
医药卫生引用本文复制引用
任朝辉,段婷婷,陆承云,张传博..蝙蝠蛾拟青霉与放线菌共培养对次生代谢产物的影响[J].天然产物研究与开发,2017,29(7):1085-1090,1147,7.基金项目
贵州省贵州师范大学研究生创新基金(2015-22) （2015-22）
国家自然科学基金(81460585) （81460585）
贵州省林业厅育林基金(2012-08) （2012-08）
贵州省社会发展科技攻关计划(2013-3154) （2013-3154）
