天然产物研究与开发2017，Vol.29Issue(7)：1102-1106,5.DOI:10.16333/j.1001-6880.2017.7.004
伪原薯蓣皂苷对Ox-LDL诱导人脐静脉内皮细胞COX-2表达的影响及机制研究
Effect of Pseudoprotodioscin on OX-LDL-induced COX-2 Expression in HUVEC
摘要
Abstract
Pseudoprotodioscinis the main active compound of Di'aoXinXue Kang capsules.To explore the effect of pseudoprotodioscin on cyclooxygenase-2 (COX-2) expression induced by OX-LDL in human umbilical vein endothelial cells (HUVEC),HUVEC cells were cultured in vitro.After pretreatment with different concentrations of pseudoprotodioscin for 24 h,OX-LDL was added for the induction of COX-2 expression.The expressions of COX-2 mRNA was detected by Real-time PCR,and the expression of COX-2 protein and the phosphorylation of mitogen activated protein kinase (MAPK) associated with COX-2 inflammatory signaling pathway were determined by Western blot.Our results showed that pseudoprotodioscin can inhibit OX-LDL-induced COX-2 expression in HUVEC dose-dependently,and inhibit TLR2 expression and p38MAPK activity.These results indicated that pseudoprodioscininhibited OX-LDL-induced COX-2 expression possibly via affecting the TLR2 / p38MAPK pathway,suggesting its anti-inflammatory effect on vein endothelial cells.关键词
伪原薯蓣皂苷/低密度氧化脂蛋白/人脐静脉内皮细胞/环氧合酶-2/TLR2/p38MAPK通路Key words
Pseudoprotodioscin/OX-LDL/human umbilical vein endothelial cells/cyclooxygenase-2/TLR2/P38MAPK pathway分类
医药卫生引用本文复制引用
李迪迪,陈扬,谭婉贤,邹文俊..伪原薯蓣皂苷对Ox-LDL诱导人脐静脉内皮细胞COX-2表达的影响及机制研究[J].天然产物研究与开发,2017,29(7):1102-1106,5.基金项目
国家科技部“十二五”重大新药创制专项(2012ZX09101231) （2012ZX09101231）
