天然产物研究与开发2017，Vol.29Issue(7)：1113-1118,6.DOI:10.16333/j.1001-6880.2017.7.006
淫羊藿苷对小鼠黑色素瘤B16细胞增殖、凋亡及迁移的影响
Effects of Icariin on Proliferation, Apoptosis and Migration of Mouse Melanoma B16 Cells
摘要
Abstract
To explore the effects of icariin on cell proliferation,apoptosis and migration of mouse melanoma B16 cells.After treatment with different concentrations of icariin,B16 cell proliferation inhibition rate was detected by MTT assay.The morphologic changes in B16 cells was observed after Hoechst 33258 staining.Cell apoptosis was evaluated by Annexin Ⅴ-FITC/PI double staining.The protein levels of Bax,Bcl-2 and Caspase-3 in icariin-treated B16 cells were examined by western blot.Moreover,cell migration activity of icariin-treated B16 cells was examined by scratch wound assay and transwell migration assay.Compared with the control group,icariin significantly inhibited the proliferation of B16 cells in a concentration manner.Morphological changes including chromatic agglutination and nuclear fragmentation were observed in icariin-treated B16 cells.Meanwhile,the apoptosis rate of B16 cells was remarkably increased after icariin treatment.Western blot analysis showed that icariin up-regulated the protein levels of Bax and Caspase-3 and down-regulated the Bcl-2 protein level.In addition,icariin inhibited the migration of B16 cells in a concentration-dependent manner.Therefore,icariin can effectively inhibit cell proliferation,induce cell apoptosis and inhibit cell migration in B16 cells.关键词
淫羊藿苷/B16细胞/增殖/迁移/凋亡Key words
icariin/B16 cells/proliferation/migration/apoptosis分类
医药卫生引用本文复制引用
王丹,徐文娟,叶蕾,李德芳,高彩霞,于丽娜,郑秋生..淫羊藿苷对小鼠黑色素瘤B16细胞增殖、凋亡及迁移的影响[J].天然产物研究与开发,2017,29(7):1113-1118,6.基金项目
滨州医学院科研启动基金(BY2014KYQD01) （BY2014KYQD01）
新疆生产建设兵团重点领域创新团队(2015BD005) （2015BD005）
国家自然科学基金(31471338 （）
81602556) （）
科技型中小企业创业投资引导基金(13C26216516410) （13C26216516410）
山东省高等学校优势学科人才团队培育计划 （）
