天然产物研究与开发2017，Vol.29Issue(7)：1119-1126,8.DOI:10.16333/j.1001-6880.2017.7.007
有氧运动加螺旋藻补充对2型糖尿病大鼠肾脏TLR4/NF-κB-p65的表达及炎性因子的影响
Effects of Aerobic Exercise plus Spirulina Supplement on the Expression of TLR4/NF-κB-p65 and Inflammatory Factor in Kidney Tissue of Type 2 Diabetes Rats
摘要
Abstract
To investigate the effects and possible mechanism of aerobic exercise and spirulina supplement on the protein expression of TLR4/NF-κB-p65 as a target of improving renal injury and inflammatory factor in kidney tissue of type 2 diabetic rats.The model of type 2 diabetic rats was established through SD rats fed high-fat diet for four weeks together with intraperitoneal infecting a low dose of STZ.The model rats were randomly divided into diabetic control group(DM) and diabetic exercise group (DE) with intervention of swimming training for eight weeks,and diabetic plus spirulina group(DS) and diabetic exercise plus spirulina group(DES),additionally provided normal control group (NC) without any intervention.The blood glucose concentrations and the excretion of 24 h microalbuminuria(UAE) were examined,and the expressions of TNF-α,TLR4 and NF-κB-p65 in the renal cortex and glomerular microstructure changes were observed by light microscope after the experiment end.The results indicated that group DM had pathological changes of glomerular capillary loop increased and dilation of renal tubule and its epithelial cell epithelial and degeneration.In addition,cell aggregation increased and infiltration of inflammatory cells,while the above glomerular injury improved significantly in groups of DE,DS and DES with exercise and spirulina supplement through light microscope.Compared with group DM,the concentrations of blood glucose and 24h UAE in the intervention groups decreased significantly(P ＜ 0.05 or P ＜ 0.01),and that improved significantly after two interventions than the single intervention.Compared with group DM,the expressions of NF-κB-p65 and TNF-α,TLR4 significantly decreased in group DE of the renal cortex(P ＜0.05 or P ＜ 0.01),and that improved significantly after two interventions than the single interventions.Therefore,exercise and/or spirulina supplement can improve blood glucose and renal function,and decrease renal injure for type 2 diabetes.Combination of swimming and spirulina supplement showed stronger effects on protein expression of TLR4/NF-κB-p65 signal which had anti-inflammatory effect than either sole swimming or sole spirulina supplement.关键词
2型糖尿病/游泳运动/螺旋藻/肾脏损伤/TLR4/NF-κB-p65Key words
type 2 diabetes/exercise training/renal injury/TLR4/NF-κB-p65/spirulina分类
医药卫生引用本文复制引用
朱洪竹,朱梅菊,曾志刚,丁孝民,伍人乐..有氧运动加螺旋藻补充对2型糖尿病大鼠肾脏TLR4/NF-κB-p65的表达及炎性因子的影响[J].天然产物研究与开发,2017,29(7):1119-1126,8.基金项目
国家自然科学基金(31360255) （31360255）
江西省教育厅科学技术研究项目(GJJ150770) （GJJ150770）
井冈山大学自然科学研究项目(JZB1313) （JZB1313）
