天然产物研究与开发2017，Vol.29Issue(7)：1127-1131,5.DOI:10.16333/j.1001-6880.2017.7.008
血根碱通过下调TGF-β1/Smad通路抑制紫杉醇耐药卵巢癌细胞生长
Sanguinarine Inhibits the Growth of Paclitaxel Resistant Ovarian Cells by Down-Regulating the TGF-β1/Smad Pathway
摘要
Abstract
Paclitaxel resistant ovarian cancer A2780/Taxol cells were divided into control group and sanguinarine group.The cells in control group were treated without sanguinarine.The cells in sanguinarine group were treated with different concentrations of sanguinafine (0.67 μmol/L,1.0 μmol/L,2.0 μmol/L).The proliferation,apoptosis,cycle and the expression of Bax,TGF-beta 1,Smad3 were determined by MTT,clone formation,flow cytometry and Western blot assays.It was found that the proliferation of A2780/Taxol cells were significantly inhibited by 2.0 μmol/L of sanguinarine.Compared to the control group,the expressions of Bax protein increased and TGF-β1,Smad3 protein decreased (P＜ 0.05).Hence,it was concluded that sanguinarine can effectively inhibit the growth of A2780/Taxol cells by downregulating the TGF-β1/Smad3 pathway and inducing cell apoptosis.关键词
血根碱/卵巢癌/紫杉醇耐药/TGF-β1/SmadKey words
sanguinarine/ovarian cancer/paclitaxel resistance/TGF-β1/Smad分类
医药卫生引用本文复制引用
王妍妍,张琴,冷天艳,杨丽华..血根碱通过下调TGF-β1/Smad通路抑制紫杉醇耐药卵巢癌细胞生长[J].天然产物研究与开发,2017,29(7):1127-1131,5.基金项目
云南省卫生和计划生育委员会医学学科带头人培养项目(D-201633) （D-201633）
国家自然科学基金地区(81360336) （81360336）
云南省科技厅-昆明医科大学应用基础研究联合专项资金项目 （）
