天然产物研究与开发2017，Vol.29Issue(7)：1148-1153,6.DOI:10.16333/j.1001-6880.2017.7.012
金花茶叶多糖的分离纯化及组成分析
Extraction, Purification and Component Analysis of Polysaccharide from Camellia chrysantha (Hu) Tuyama Leaves
摘要
Abstract
Crude polysaccharides (CCTLP) were extracted from dry leaves of Camellia chrysantha (Hu)Tuyama using water.The four parts including CCTLP-0,CCTLP-0.15,CCTLP-0.3 and CCTLP-0.45 were obtained from CCTLP through sequentially gradient elution by water,0.15 mol/L NaCl,0.3 mol/L NaCl and 0.45 mol/L NaCl as elution reagent by DEAE-cellulose column after removing pigments of CCTLP by D101 macroporous resin.CCTLP-0 and CCTLP-0.15 were then deproteinized by Sevage method,running water dialysis and removing residual NaCl by Sephadex G-15 chromatography for further purification.The five fractions including CCTLP-0-1,CCTLP-0.15-1,CCTLP-0.15-2,CCTLP0.15-3 and CCTLP-0.15-0.4 were obtained by purification from CCTLP-0 and CCTLP-0.15 using agarose Sepharose 6FF column chromatography.The results showed that they were homogeneous polysaccharide through GPC detection in molecular weight and molecular weight distribution and in analysis of GPC peak type detected.Both CCTLP-0 and CCTLP-0.15 were mainly composted of rhamnose,fructose and galactose through HPLC analysis after they were acid hydrolysis,which molar mass ratios were similar to 1.00∶ 1.066∶ 0.384 and 1.00∶ 1.186∶ 0.544,respectively.关键词
金花茶叶多糖/DEAE-纤维素分离/凝胶色谱纯化/GPC检测/多糖分析Key words
polysachaide from Camellia chrysantha (Hu) Tuyama leaves/DEAE-cellulose column separation/gel permeation chromatography purification/GPC detection/polysaccharide analysis分类
化学化工引用本文复制引用
许子竞,石谦,罗树常..金花茶叶多糖的分离纯化及组成分析[J].天然产物研究与开发,2017,29(7):1148-1153,6.基金项目
贵州省科技厅、毕节市科技局、贵州工程应用技术学院联合基金(LH-2016-7059) （LH-2016-7059）
贵州省毕节市2016年科学发展计划(2016-32) （2016-32）
