天然产物研究与开发2017，Vol.29Issue(7)：1182-1187,1153,7.DOI:10.16333/j.1001-6880.2017.7.018
六妹羊肚菌多糖部分理化性质及抗氧化活性研究
Physical and Chemical Properties of Polysaccharide Isolated from the Fruiting Bodies of Morchella sextelata and its Antioxidant Effect
摘要
Abstract
This article mainly studied the extraction,purification,structure and antioxidant effects of a polysaccharide (MSP) purified from the fruiting bodies of Morchella sextelata M.Kuo.The polysaccharides of M.sextelata were extracted with boiling water and purified by DE-52 column and Sephadex G-100 column.The molecular weight,monosaccharide composition were analyzed by HPGPC and HPLC.In order to investigate the neuroprotective role of MSP,the antioxidant activity of MSP against the H2 O2-induced cytotoxicity and the underlying preventative mechanisms in rat pheochromocytoma (PC12) cells were illustrated.It was shown that the molecular weight of MSP was 287 588 Da(287 588 Da),the monosaccharide composition of MSPwasmainly composed of mannose,glucose and galactose,with a ratio near 9∶ 1∶6.MSP can considerably enhance the viability of PC12 cells exposure to H2O2 and increase the activities of antioxidant enzyme like SOD,CAT and GSH-Px.It inhibited cell apoptosis via reducing caspase-3 activation and down-regulation Bax/Bcl-2 ratio.Accordingly,MSP can be used as a promising neuroprotective compound for nervous diseases treatment.关键词
六妹羊肚菌/多糖提取/PC12细胞/抗氧化/神经保护Key words
Morchella sextelata/extraction of polysaccharide/PC-12 cell/antioxidant/neuroprotective分类
生物科学引用本文复制引用
熊川,罗强,李强,陈诚,陈祖琴,黄文丽..六妹羊肚菌多糖部分理化性质及抗氧化活性研究[J].天然产物研究与开发,2017,29(7):1182-1187,1153,7.基金项目
四川省科技支撑计划(2016GZ0355) （2016GZ0355）
