天然产物研究与开发2017，Vol.29Issue(7)：1204-1209,6.DOI:10.16333/j.1001-6880.2017.7.022
十子代平方对T2DM大鼠骨骼肌PI3K、GLUT4蛋白表达的影响
Effects of Shizidaiping on PI3K, P-PI3K and GLUT4 Expression in Musculi Skeleti of Type 2 Diabetic Rats
摘要
Abstract
The aim of this study was to explore the improvement function of Shizidaiping on insulin resistance of type 2 diabetic SD rats model and the influence on the expression of musculi skeleti insulin signal transduction proteins PI3K,P-PI3K and GLUT4 as well as to provide experimental basis for the clinical application of Shizidaiping.Breeding induced SD rats with type 2 diabetes insulin resistance model was developed using streptozotocin,high fat and sugar.Practice lavage intervention on model rats with high,medium and low doses of Shizidaiping and set up normal control group,model group and metformin group.Eight weeks later,practice normal saline lavage for normal group and model group.Fasting blood glucose and glucose tolerance test were carried out before using the medicine,after 4 and 8 weeks of using medicine.After 8 weeks of using the medicine,glycosylated hemoglobin (GHB),glycosylated serum protein (gsp) and cpeptide (c-p) were examined.Insulin resistance index and protein expression level of musculi skelet were calculated.Then statistical analysis was carried out using SPSS 20.0 software.Compared the treatment groups with the model groups after 8 weeks of using the medicine,it was found that FBG,PG2h and AUC decreased significantly (P ＜ 0.05 or P ＜ 0.01).After the treatment finished,there was obvious difference between the treatment groups and the model groups on GHB,GSP,C-P and HOMA-IR (P ＜0.05 or P ＜0.01).There was no obvious difference between different groups on musculi skeleti PI3K (P ＞ 0.05).The protein expression level of P-PI3K and GLUT4 of the treatment groups was significantly higher than these of model groups (P ＜ 0.05).Therefore,Shizidaiping can reduce blood glucose of type 2 diabetic rats and improve insulin resistance (IR).Its pharmacological mechanism was possibly through improving of protein expression level of P-PI3K and GLUT4 of musculi skeleti.关键词
十子代平方/糖代谢/骨骼肌胰岛素抵抗/PI3K/GLUT4Key words
Shizidaiping/glycometabolism/musculi skeleti insulin resistance/PI3K/GLUT4分类
医药卫生引用本文复制引用
张碧溦,田春雨,喇孝瑾,徐雪梅,焦子珊,林椠,王晨斌,李继安..十子代平方对T2DM大鼠骨骼肌PI3K、GLUT4蛋白表达的影响[J].天然产物研究与开发,2017,29(7):1204-1209,6.基金项目
河北省国际科技合作计划(13392502D) （13392502D）
河北省中医药管理局科研计划(2014189) （2014189）
河北省自然科学基金(H2015209025) （H2015209025）
河北省高等学校科学技术研究项目(QN2015119) （QN2015119）
